Untargeted Urinary Volatilomics Reveals Hexadecanal as a Potential Biomarker for Preeclampsia

Preeclampsia (PE) is a severe hypertensive pregnancy disorder characterized by endothelial dysfunction, placental ischemia and oxidative stress; however, reliable non-invasive biomarkers for early detection are limited. In this study, untargeted solid-phase microextraction with gas chromatography-ma...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-11, Vol.25 (22), p.12371
Main Authors: Pehlić, Marina, Dumančić, Stipe, Radan, Mila, Galić, Jelena, Gruica, Branimir, Marijan, Sandra, Vulić, Marko
Format: Article
Language:English
Subjects:
Adult
Aldehydes - analysis
Aldehydes - metabolism
Aldehydes - urine
Biomarkers
Biomarkers - urine
Case-Control Studies
Female
Gas Chromatography-Mass Spectrometry - methods
Humans
Hypertension
Ischemia
Lipid peroxidation
Lipids
Mass spectrometry
Metabolites
Oxidative Stress
Pre-Eclampsia - diagnosis
Pre-Eclampsia - metabolism
Pre-Eclampsia - urine
Preeclampsia
Pregnancy
ROC Curve
Solid Phase Microextraction - methods
Statistical analysis
Volatile organic compounds
Volatile Organic Compounds - analysis
Volatile Organic Compounds - metabolism
Volatile Organic Compounds - urine
Womens health
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Summary:Preeclampsia (PE) is a severe hypertensive pregnancy disorder characterized by endothelial dysfunction, placental ischemia and oxidative stress; however, reliable non-invasive biomarkers for early detection are limited. In this study, untargeted solid-phase microextraction with gas chromatography-mass spectrometry (SPME-GC-MS) was used to analyze volatile organic compounds in the urine of 45 women with PE and 46 healthy controls. Among the 29 metabolites identified, hexadecanal-a product of lipid peroxidation and sphingolipid metabolism-was found to be the most significant, with an area under the receiver operating characteristic (ROC) curve of 0.618, highlighting its diagnostic potential. This result emphasizes the role of hexadecanal in oxidative stress and placental dysfunction, which are central to the pathophysiology of PE. The results support hexadecanal as a potential non-invasive biomarker while demonstrating the efficacy of SPME-GC-MS in identifying metabolic disorders associated with PE, paving the way for further research to confirm its clinical utility for early diagnosis and risk assessment.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms252212371