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Relationship between cell surface carbohydrates and intrastrain variation on opsonophagocytosis of Streptococcus pneumoniae

Streptococcus pneumoniae undergoes spontaneous phase variation between a transparent and an opaque colony phenotype, the latter being more virulent in a murine model of sepsis. Opaque pneumococci have previously been shown to express lower amounts of C polysaccharide (cell wall teichoic acid) and in...

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Published in:Infection and immunity 1999-05, Vol.67 (5), p.2327-2333
Main Authors: KIM, J. O, ROMERO-STEINER, S, SØRENSEN, U. B. S, BLOM, J, CARVALHO, M, BARNARD, S, CARLONE, G, WEISER, J. N
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cited_by cdi_FETCH-LOGICAL-c446t-918d4c820ddfb0e7060e6fc56ac0e306f34a81c93e8c86ade7a4737f69be0b5d3
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container_title Infection and immunity
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creator KIM, J. O
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description Streptococcus pneumoniae undergoes spontaneous phase variation between a transparent and an opaque colony phenotype, the latter being more virulent in a murine model of sepsis. Opaque pneumococci have previously been shown to express lower amounts of C polysaccharide (cell wall teichoic acid) and in this study were shown to have a higher content of capsular polysaccharide by immunoelectron microscopy. This report then examined the relationship between expression of these two cell surface carbohydrate structures and their relative contribution to the increased virulence of opaque variants. Comparison of genetically related strains showed that the differential content of capsular polysaccharide did not affect the amount of teichoic acid as measured by a capture enzyme-linked immunosorbent assay (ELISA). In contrast, when the teichoic acid structure was altered by replacing choline in the growth medium with structural analogs, the quantity of capsular polysaccharide as measured by a capture ELISA was decreased, demonstrating a linkage in the expression of the two surface carbohydrate structures. A standardized assay was used to assess the relative contribution of cell surface carbohydrates to opsonophagocytosis. The opaque variants required 1.2- to 30-fold more immune human serum to achieve 50% opsonophagocytic killing than did related transparent variants (types 6B and 9V). The opsonophagocytic titer was proportional to the quantity of capsular polysaccharide rather than teichoic acid. The major factor in binding of the opsonin, C-reactive protein (CRP), was also the amount of capsular polysaccharide rather than the teichoic acid ligand. Only for the transparent variant (type 6B), which bound more CRP, was there enhanced opsonophagocytic killing in the presence of this serum protein. Increased expression of capsular polysaccharide, therefore, appeared to be the major factor in the decreased opsonophagocytic killing of opaque pneumococci.
doi_str_mv 10.1128/IAI.67.5.2327-2333.1999
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ispartof Infection and immunity, 1999-05, Vol.67 (5), p.2327-2333
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source American Society for Microbiology Journals; PubMed Central
subjects Animals
Antibodies, Bacterial
Bacteriology
Biological and medical sciences
C-Reactive Protein - metabolism
Carbohydrates - immunology
Cell Membrane - immunology
Fundamental and applied biological sciences. Psychology
Genetic Variation
HL-60 Cells
Humans
Mice
Microbiology
Microscopy, Immunoelectron
Molecular and Cellular Pathogenesis
Opsonin Proteins
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Phagocytosis
Phenotype
Polysaccharides, Bacterial - immunology
Streptococcus pneumoniae - immunology
Streptococcus pneumoniae - pathogenicity
Streptococcus pneumoniae - ultrastructure
Teichoic Acids - immunology
Virulence - immunology
title Relationship between cell surface carbohydrates and intrastrain variation on opsonophagocytosis of Streptococcus pneumoniae
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