Intracellular Calcium Dynamics in Primary Human Adrenocortical Cells Deciphered with a Novel Pipeline

IntroductionThe fluctuations of the intracellular Ca2+ concentration ([Ca2+]i) are key physiological signals for cell function under normal conditions and can undergo profound alterations in disease states, as high blood pressure due to endocrine disorders like primary aldosteronism (PA). However, w...

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Bibliographic Details
Published in:High blood pressure & cardiovascular prevention 2024-05, Vol.31 (3), p.299-308
Main Authors: Ajjour, Hala, Pallafacchina, Giorgia, Lenzini, Livia, Caroccia, Brasilina, Rossi, Gian Paolo
Format: Article
Language:English
Subjects:
Algorithms
Blood pressure
Cells
Data processing
Data smoothing
Gene expression
Kinases
Original
Physiology
Software
Online Access:Get full text
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Summary:IntroductionThe fluctuations of the intracellular Ca2+ concentration ([Ca2+]i) are key physiological signals for cell function under normal conditions and can undergo profound alterations in disease states, as high blood pressure due to endocrine disorders like primary aldosteronism (PA). However, when assessing such fluctuations several parameters in the Ca2+ signal dynamics need to be considered, which renders their assessment challenging.Aim Aim to develop an observer-independent custom-made pipeline to analyze Ca2+ dynamics in terms of frequency and peak parameters, as amplitude, full width at half maximum (FWHM) and area under the curve (AUC).MethodsWe applied a custom-made methodology to aldosterone-producing adenoma (APA) and APA adjacent cells (AAC) and found this pipeline to be suitable for monitoring and processing a wide-range of [Ca2+]i events in these cell types delivering reproducible results.ConclusionThe designed pipeline can provide a useful tool for [Ca2+]i signal analysis that allows comparisons of Ca2+ dynamics not only in PA, but in other cell phenotypes that are relevant for the regulation of blood pressure.
ISSN:1120-9879
1179-1985
DOI:10.1007/s40292-024-00641-5