Reduction-responsive immobilised and protected enzymes

We report a synthetic strategy to produce nano-immobilised and organosilica-shielded enzymes of which the biocatalytic activity is, by design, chemically enhanced under reductive conditions. The enzymes were immobilised onto silica nanoparticles through a reduction-responsive crosslinker and further...

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Bibliographic Details
Published in:Nanoscale advances 2024-12, Vol.7 (1), p.89-93
Main Authors: Wu, Congyu, Nazemi, Seyed Amirabbas, Santacroce, Natascha, Sahlin, Jenny A, Suter-Dick, Laura, Shahgaldian, Patrick
Format: Article
Language:English
Subjects:
Chemistry
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Summary:We report a synthetic strategy to produce nano-immobilised and organosilica-shielded enzymes of which the biocatalytic activity is, by design, chemically enhanced under reductive conditions. The enzymes were immobilised onto silica nanoparticles through a reduction-responsive crosslinker and further shielded in an organosilica layer of controlled thickness. Under reducing conditions, disulphide bonds linking the protein to the carrier material were reduced, triggering enzyme activation. The organosilica shield prevents the enzymes from leaching from the nanobiocatalysts and preserves their integrity. Enzymes unchained - enzymes immobilised on nanoparticles with a cleavable linker and shielded in an organosilica layer are activated upon reductive breakage of the linker. The organosilica layer prevents enzyme leaching.
ISSN:2516-0230
2516-0230
DOI:10.1039/d4na00580e