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Nuclear Expression of Dynamin 2 Is Associated With Tumor Aggressiveness in Bladder Cancer Patients: A Bioinformatics and Experimental Approach
ABSTRACT Background Dynamin 2 (DNM2) is aberrantly expressed in different malignancies and exerts a function in tumor progression. Aims This study, for the first time, aimed to evaluate the clinical and prognostic value of DNM2 in the pathophysiology of bladder cancer using bioinformatics analysis a...
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| Published in: | Cancer reports 2024-12, Vol.7 (12), p.e2133-n/a |
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| creator | Razmi, Mahdieh Saeednejad Zanjani, Leili Rahimi, Mandana Sajed, Roya Safaei, Sadegh Madjd, Zahra Ghods, Roya |
| description | ABSTRACT
Background
Dynamin 2 (DNM2) is aberrantly expressed in different malignancies and exerts a function in tumor progression.
Aims
This study, for the first time, aimed to evaluate the clinical and prognostic value of DNM2 in the pathophysiology of bladder cancer using bioinformatics analysis and experimental evaluation.
Methods and Results
We analyzed gene expression of DNM2 in bladder tumor by GEPIA2 and GENT2 platforms. Cluster subnetworks were recognized from the protein–protein interaction (PPI) network using the MCODE plugin to screen the key genes. Subsequently, the pathway enrichment analysis was evaluated. Then, the immunohistochemical examination was conducted on 209 paraffin‐embedded bladder cancer samples to determine the expression pattern and clinical importance of DNM2. Our data mining findings demonstrated dysregulation of DNM2 gene expression in bladder cancer. The results of pathway and PPI network analyses indicated that DNM2 might be involved in the development of bladder cancer by influencing various signaling pathways. Our IHC results represented remarkably higher DNM2 expression in bladder tumor samples compared to normal tissue samples adjacent to tumor. A statistically significant association was identified between DNM2 expression in the nucleus and higher histological grade (p = 0.026), advanced pT stage (p = 0.016), muscular invasion (p = 0.007), tumor recurrence (p = 0.030), and distant metastasis (p |
| doi_str_mv | 10.1002/cnr2.2133 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11604598</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_6a8406125c004d048d38fd9ed34142eb</doaj_id><sourcerecordid>3134068625</sourcerecordid><originalsourceid>FETCH-LOGICAL-d3033-f8be7f6c2bbfa716946cd1b490710a69c8d730418d7ed722d3a4f110e9af22be3</originalsourceid><addsrcrecordid>eNpVUstu1DAUjRCIVqULfgB5yWZav8Zx2KB0WmCkqiBUxNK6sZ0ZV4kd7KQwX8Ev8C18GU6nVO3qXN177jnH8i2K1wSfEIzpqfaRnlDC2LPikC5LtpCMy-eP6oPiOKUbjDGRgtGKvSwOWCXyLq4Oi99Xk-4sRHTxa4g2JRc8Ci0633nonUcUrROqUwrawWgN-u7GLbqe-hBRvdncLdxanwFl8lkHxtiIVuB1hi8wOuvH9A7Vf_-cueB8G2Kfmzoh8GZ2tNH1mQIdqochBtDbV8WLFrpkj-_xqPj24eJ69Wlx-fnjelVfLgzDjC1a2diyFZo2TQslERUX2pCGV7gkGESlpSkZ5iSDNSWlhgFvCcG2gpbSxrKjYr3XNQFu1JBzQNypAE7dNULcKIg5ameVAMmxIHSpMeYGc2mYbE1lDeOEU9tkrfd7rWFqemt0flGE7ono04l3W7UJt4oQgfmyklnh7b1CDD8mm0bVu6Rt14G3YUqKEZYjSEGXmfrmsdmDy_8vzYTTPeGn6-zuYU6wms9Fzeei5nNRq6uvdC7YPwI-tGE</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3134068625</pqid></control><display><type>article</type><title>Nuclear Expression of Dynamin 2 Is Associated With Tumor Aggressiveness in Bladder Cancer Patients: A Bioinformatics and Experimental Approach</title><source>Wiley Online Library Journals Open Access</source><source>Publicly Available Content Database</source><source>Wiley-Blackwell Read & Publish Collection</source><source>PubMed Central</source><creator>Razmi, Mahdieh ; Saeednejad Zanjani, Leili ; Rahimi, Mandana ; Sajed, Roya ; Safaei, Sadegh ; Madjd, Zahra ; Ghods, Roya</creator><creatorcontrib>Razmi, Mahdieh ; Saeednejad Zanjani, Leili ; Rahimi, Mandana ; Sajed, Roya ; Safaei, Sadegh ; Madjd, Zahra ; Ghods, Roya</creatorcontrib><description>ABSTRACT
Background
Dynamin 2 (DNM2) is aberrantly expressed in different malignancies and exerts a function in tumor progression.
Aims
This study, for the first time, aimed to evaluate the clinical and prognostic value of DNM2 in the pathophysiology of bladder cancer using bioinformatics analysis and experimental evaluation.
Methods and Results
We analyzed gene expression of DNM2 in bladder tumor by GEPIA2 and GENT2 platforms. Cluster subnetworks were recognized from the protein–protein interaction (PPI) network using the MCODE plugin to screen the key genes. Subsequently, the pathway enrichment analysis was evaluated. Then, the immunohistochemical examination was conducted on 209 paraffin‐embedded bladder cancer samples to determine the expression pattern and clinical importance of DNM2. Our data mining findings demonstrated dysregulation of DNM2 gene expression in bladder cancer. The results of pathway and PPI network analyses indicated that DNM2 might be involved in the development of bladder cancer by influencing various signaling pathways. Our IHC results represented remarkably higher DNM2 expression in bladder tumor samples compared to normal tissue samples adjacent to tumor. A statistically significant association was identified between DNM2 expression in the nucleus and higher histological grade (p = 0.026), advanced pT stage (p = 0.016), muscular invasion (p = 0.007), tumor recurrence (p = 0.030), and distant metastasis (p < 0.001). Moreover, the nuclear DNM2 expression was observed to have prognostic significance for disease‐specific survival (DSS) using a log‐rank test (p = 0.028).
Conclusion
These findings suggest that nuclear DNM2 expression could be a putative indicator of bladder tumor progression owing to its association with elevated cancer aggressiveness.</description><identifier>ISSN: 2573-8348</identifier><identifier>EISSN: 2573-8348</identifier><identifier>DOI: 10.1002/cnr2.2133</identifier><identifier>PMID: 39610009</identifier><language>eng</language><publisher>United States: John Wiley and Sons Inc</publisher><subject>Aged ; bioinformatics ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; bladder cancer (BC) ; Cell Nucleus - metabolism ; Computational Biology ; dynamin 2 (DNM2) ; Dynamin II - genetics ; Dynamin II - metabolism ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; immunohistochemistry (IHC) ; Male ; Middle Aged ; Original ; Prognosis ; Protein Interaction Maps ; tissue microarray (TMA) ; Urinary Bladder Neoplasms - genetics ; Urinary Bladder Neoplasms - metabolism ; Urinary Bladder Neoplasms - mortality ; Urinary Bladder Neoplasms - pathology</subject><ispartof>Cancer reports, 2024-12, Vol.7 (12), p.e2133-n/a</ispartof><rights>2024 The Author(s). published by Wiley Periodicals LLC.</rights><rights>2024 The Author(s). Cancer Reports published by Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0001-5996-3758 ; 0000-0002-6257-6852</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11604598/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11604598/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,11562,27924,27925,37013,46052,46476,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39610009$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Razmi, Mahdieh</creatorcontrib><creatorcontrib>Saeednejad Zanjani, Leili</creatorcontrib><creatorcontrib>Rahimi, Mandana</creatorcontrib><creatorcontrib>Sajed, Roya</creatorcontrib><creatorcontrib>Safaei, Sadegh</creatorcontrib><creatorcontrib>Madjd, Zahra</creatorcontrib><creatorcontrib>Ghods, Roya</creatorcontrib><title>Nuclear Expression of Dynamin 2 Is Associated With Tumor Aggressiveness in Bladder Cancer Patients: A Bioinformatics and Experimental Approach</title><title>Cancer reports</title><addtitle>Cancer Rep (Hoboken)</addtitle><description>ABSTRACT
Background
Dynamin 2 (DNM2) is aberrantly expressed in different malignancies and exerts a function in tumor progression.
Aims
This study, for the first time, aimed to evaluate the clinical and prognostic value of DNM2 in the pathophysiology of bladder cancer using bioinformatics analysis and experimental evaluation.
Methods and Results
We analyzed gene expression of DNM2 in bladder tumor by GEPIA2 and GENT2 platforms. Cluster subnetworks were recognized from the protein–protein interaction (PPI) network using the MCODE plugin to screen the key genes. Subsequently, the pathway enrichment analysis was evaluated. Then, the immunohistochemical examination was conducted on 209 paraffin‐embedded bladder cancer samples to determine the expression pattern and clinical importance of DNM2. Our data mining findings demonstrated dysregulation of DNM2 gene expression in bladder cancer. The results of pathway and PPI network analyses indicated that DNM2 might be involved in the development of bladder cancer by influencing various signaling pathways. Our IHC results represented remarkably higher DNM2 expression in bladder tumor samples compared to normal tissue samples adjacent to tumor. A statistically significant association was identified between DNM2 expression in the nucleus and higher histological grade (p = 0.026), advanced pT stage (p = 0.016), muscular invasion (p = 0.007), tumor recurrence (p = 0.030), and distant metastasis (p < 0.001). Moreover, the nuclear DNM2 expression was observed to have prognostic significance for disease‐specific survival (DSS) using a log‐rank test (p = 0.028).
Conclusion
These findings suggest that nuclear DNM2 expression could be a putative indicator of bladder tumor progression owing to its association with elevated cancer aggressiveness.</description><subject>Aged</subject><subject>bioinformatics</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>bladder cancer (BC)</subject><subject>Cell Nucleus - metabolism</subject><subject>Computational Biology</subject><subject>dynamin 2 (DNM2)</subject><subject>Dynamin II - genetics</subject><subject>Dynamin II - metabolism</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>immunohistochemistry (IHC)</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Original</subject><subject>Prognosis</subject><subject>Protein Interaction Maps</subject><subject>tissue microarray (TMA)</subject><subject>Urinary Bladder Neoplasms - genetics</subject><subject>Urinary Bladder Neoplasms - metabolism</subject><subject>Urinary Bladder Neoplasms - mortality</subject><subject>Urinary Bladder Neoplasms - pathology</subject><issn>2573-8348</issn><issn>2573-8348</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>DOA</sourceid><recordid>eNpVUstu1DAUjRCIVqULfgB5yWZav8Zx2KB0WmCkqiBUxNK6sZ0ZV4kd7KQwX8Ev8C18GU6nVO3qXN177jnH8i2K1wSfEIzpqfaRnlDC2LPikC5LtpCMy-eP6oPiOKUbjDGRgtGKvSwOWCXyLq4Oi99Xk-4sRHTxa4g2JRc8Ci0633nonUcUrROqUwrawWgN-u7GLbqe-hBRvdncLdxanwFl8lkHxtiIVuB1hi8wOuvH9A7Vf_-cueB8G2Kfmzoh8GZ2tNH1mQIdqochBtDbV8WLFrpkj-_xqPj24eJ69Wlx-fnjelVfLgzDjC1a2diyFZo2TQslERUX2pCGV7gkGESlpSkZ5iSDNSWlhgFvCcG2gpbSxrKjYr3XNQFu1JBzQNypAE7dNULcKIg5ameVAMmxIHSpMeYGc2mYbE1lDeOEU9tkrfd7rWFqemt0flGE7ono04l3W7UJt4oQgfmyklnh7b1CDD8mm0bVu6Rt14G3YUqKEZYjSEGXmfrmsdmDy_8vzYTTPeGn6-zuYU6wms9Fzeei5nNRq6uvdC7YPwI-tGE</recordid><startdate>202412</startdate><enddate>202412</enddate><creator>Razmi, Mahdieh</creator><creator>Saeednejad Zanjani, Leili</creator><creator>Rahimi, Mandana</creator><creator>Sajed, Roya</creator><creator>Safaei, Sadegh</creator><creator>Madjd, Zahra</creator><creator>Ghods, Roya</creator><general>John Wiley and Sons Inc</general><general>Wiley</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-5996-3758</orcidid><orcidid>https://orcid.org/0000-0002-6257-6852</orcidid></search><sort><creationdate>202412</creationdate><title>Nuclear Expression of Dynamin 2 Is Associated With Tumor Aggressiveness in Bladder Cancer Patients: A Bioinformatics and Experimental Approach</title><author>Razmi, Mahdieh ; Saeednejad Zanjani, Leili ; Rahimi, Mandana ; Sajed, Roya ; Safaei, Sadegh ; Madjd, Zahra ; Ghods, Roya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-d3033-f8be7f6c2bbfa716946cd1b490710a69c8d730418d7ed722d3a4f110e9af22be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Aged</topic><topic>bioinformatics</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>bladder cancer (BC)</topic><topic>Cell Nucleus - metabolism</topic><topic>Computational Biology</topic><topic>dynamin 2 (DNM2)</topic><topic>Dynamin II - genetics</topic><topic>Dynamin II - metabolism</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>immunohistochemistry (IHC)</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Original</topic><topic>Prognosis</topic><topic>Protein Interaction Maps</topic><topic>tissue microarray (TMA)</topic><topic>Urinary Bladder Neoplasms - genetics</topic><topic>Urinary Bladder Neoplasms - metabolism</topic><topic>Urinary Bladder Neoplasms - mortality</topic><topic>Urinary Bladder Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Razmi, Mahdieh</creatorcontrib><creatorcontrib>Saeednejad Zanjani, Leili</creatorcontrib><creatorcontrib>Rahimi, Mandana</creatorcontrib><creatorcontrib>Sajed, Roya</creatorcontrib><creatorcontrib>Safaei, Sadegh</creatorcontrib><creatorcontrib>Madjd, Zahra</creatorcontrib><creatorcontrib>Ghods, Roya</creatorcontrib><collection>Wiley Online Library Journals Open Access</collection><collection>Wiley Online Library Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Cancer reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Razmi, Mahdieh</au><au>Saeednejad Zanjani, Leili</au><au>Rahimi, Mandana</au><au>Sajed, Roya</au><au>Safaei, Sadegh</au><au>Madjd, Zahra</au><au>Ghods, Roya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nuclear Expression of Dynamin 2 Is Associated With Tumor Aggressiveness in Bladder Cancer Patients: A Bioinformatics and Experimental Approach</atitle><jtitle>Cancer reports</jtitle><addtitle>Cancer Rep (Hoboken)</addtitle><date>2024-12</date><risdate>2024</risdate><volume>7</volume><issue>12</issue><spage>e2133</spage><epage>n/a</epage><pages>e2133-n/a</pages><issn>2573-8348</issn><eissn>2573-8348</eissn><abstract>ABSTRACT
Background
Dynamin 2 (DNM2) is aberrantly expressed in different malignancies and exerts a function in tumor progression.
Aims
This study, for the first time, aimed to evaluate the clinical and prognostic value of DNM2 in the pathophysiology of bladder cancer using bioinformatics analysis and experimental evaluation.
Methods and Results
We analyzed gene expression of DNM2 in bladder tumor by GEPIA2 and GENT2 platforms. Cluster subnetworks were recognized from the protein–protein interaction (PPI) network using the MCODE plugin to screen the key genes. Subsequently, the pathway enrichment analysis was evaluated. Then, the immunohistochemical examination was conducted on 209 paraffin‐embedded bladder cancer samples to determine the expression pattern and clinical importance of DNM2. Our data mining findings demonstrated dysregulation of DNM2 gene expression in bladder cancer. The results of pathway and PPI network analyses indicated that DNM2 might be involved in the development of bladder cancer by influencing various signaling pathways. Our IHC results represented remarkably higher DNM2 expression in bladder tumor samples compared to normal tissue samples adjacent to tumor. A statistically significant association was identified between DNM2 expression in the nucleus and higher histological grade (p = 0.026), advanced pT stage (p = 0.016), muscular invasion (p = 0.007), tumor recurrence (p = 0.030), and distant metastasis (p < 0.001). Moreover, the nuclear DNM2 expression was observed to have prognostic significance for disease‐specific survival (DSS) using a log‐rank test (p = 0.028).
Conclusion
These findings suggest that nuclear DNM2 expression could be a putative indicator of bladder tumor progression owing to its association with elevated cancer aggressiveness.</abstract><cop>United States</cop><pub>John Wiley and Sons Inc</pub><pmid>39610009</pmid><doi>10.1002/cnr2.2133</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0001-5996-3758</orcidid><orcidid>https://orcid.org/0000-0002-6257-6852</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library Journals Open Access; Publicly Available Content Database; Wiley-Blackwell Read & Publish Collection; PubMed Central |
| subjects | Aged bioinformatics Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism bladder cancer (BC) Cell Nucleus - metabolism Computational Biology dynamin 2 (DNM2) Dynamin II - genetics Dynamin II - metabolism Female Gene Expression Regulation, Neoplastic Humans immunohistochemistry (IHC) Male Middle Aged Original Prognosis Protein Interaction Maps tissue microarray (TMA) Urinary Bladder Neoplasms - genetics Urinary Bladder Neoplasms - metabolism Urinary Bladder Neoplasms - mortality Urinary Bladder Neoplasms - pathology |
| title | Nuclear Expression of Dynamin 2 Is Associated With Tumor Aggressiveness in Bladder Cancer Patients: A Bioinformatics and Experimental Approach |
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