Hybrid Raman and Partial Wave Spectroscopy Microscope for the Characterization of Molecular and Structural Alterations in Tissue

ABSTRACT We present a hybrid Raman spectroscopy (RS) and partial wave spectroscopy (PWS) microscope for the characterization of molecular and structural tissue alterations. The PWS performance was assessed with surface roughness standards, while the Raman performance with a silicon crystal standard....

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Bibliographic Details
Published in:Journal of biophotonics 2024-12, Vol.17 (12), p.e202400330-n/a
Main Authors: Kriukova, Elena, Mazurenka, Mikhail, Marcazzan, Sabrina, Glasl, Sarah, Quante, Michael, Saur, Dieter, Tschurtschenthaler, Markus, Puppels, Gerwin J., Gorpas, Dimitris, Ntziachristos, Vasilis
Format: Article
Language:English
Subjects:
Animals
Carcinogenesis
Carcinogens
Chemical fingerprinting
field cancerization
Hybrid systems
Intestine
Intestines - diagnostic imaging
Intestines - pathology
light‐scattering spectroscopy
Mice
Microscopy
multimodal imaging
partial wave spectroscopy
Raman spectroscopy
Spectroscopy
Spectrum Analysis, Raman
Structural analysis
Surface roughness
Tissues
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Summary:ABSTRACT We present a hybrid Raman spectroscopy (RS) and partial wave spectroscopy (PWS) microscope for the characterization of molecular and structural tissue alterations. The PWS performance was assessed with surface roughness standards, while the Raman performance with a silicon crystal standard. We also validated the system on stomach and intestinal mouse tissues, two closely‐related tissue types, and demonstrate that the addition of PWS information improves RS data classification for these tissue types from R2 = 0.892 to R2 = 0.964 (norm of residuals 0.863 and 0.497, respectively). Then, in a proof‐of‐concept experiment, we show that the hybrid system can detect changes in intestinal tissues harvested from a tumorigenic Villin‐Cre, Apcfl/wt mouse. We discuss how the hybrid modality offers new abilities to identify the relative roles of PWS morphological features and Raman molecular fingerprints, possibly allowing for their combination to enhance the study of carcinogenesis and early cancer diagnostics in the future.
ISSN:1864-063X
1864-0648
1864-0648
DOI:10.1002/jbio.202400330