Single-Cell Analysis of Bone Marrow CD8+ T Cells in Myeloid Neoplasms Reveals Pathways Associated with Disease Progression and Response to Treatment with Azacitidine

• Published in: Cancer research communications 2024-12, Vol.4 (12), p.3067-3083
• Main Authors: Tasis, Athanasios, Papaioannou, Nikos E, Grigoriou, Maria, Paschalidis, Nikolaos, Loukogiannaki, Catherine, Filia, Anastasia, Katsiki, Kyriaki, Lamprianidou, Eleftheria, Papadopoulos, Vasileios, Rimpa, Christina Maria, Chatzigeorgiou, Antonios, Kourtzelis, Ioannis, Gerasimou, Petroula, Kyprianou, Ioannis, Costeas, Paul, Liakopoulos, Panagiotis, Liapis, Konstantinos, Kolovos, Petros, Chavakis, Triantafyllos, Alissafi, Themis, Kotsianidis, Ioannis, Mitroulis, Ioannis
• Format: Article
• Language: English
• Subjects: Aged; Antimetabolites, Antineoplastic - pharmacology; Antimetabolites, Antineoplastic - therapeutic use; Azacitidine - pharmacology; Azacitidine - therapeutic use; Bone Marrow - drug effects; Bone Marrow - pathology; CD8-Positive T-Lymphocytes - drug effects; CD8-Positive T-Lymphocytes - immunology; Disease Progression; Female; Hematological Cancers; Humans; Immunology; Leukemia, Myeloid, Acute - drug therapy; Leukemia, Myeloid, Acute - immunology; Leukemia, Myeloid, Acute - pathology; Leukemias; Male; Middle Aged; Myelodysplastic Syndromes - drug therapy; Myelodysplastic Syndromes - pathology; Signal Transduction - drug effects; Single Cell Technologies; Single-Cell Analysis
• ISSN: 2767-9764; 2767-9764
• DOI: 10.1158/2767-9764.CRC-24-0310

Immunophenotypic analysis identified a BM CD57+CXCR3+ subset of CD8+ T cells associated with response to AZA in patients with MDS and AML. Single-cell RNA sequencing analysis revealed that IFN signaling is linked to the response to treatment, whereas TGF-β signaling is associated with treatment failure, providing insights into new therapeutic approaches.