Altered lipolytic response to glucagon and adenosine deaminase in adipocytes from starved rats

1. Adipocytes isolated from epididymal adipose tissue of fed or 24 h-starved rats were incubated with a range of glucagon concentrations in the presence and absence of adenosine deaminase (4 munits/ml). 2. With adenosine deaminase present, the lipolytic response to low concentrations of glucagon (1-...

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Bibliographic Details
Published in:Biochemical journal 1980-06, Vol.188 (3), p.757-761
Main Authors: Honnor, R C, Saggerson, E D
Format: Article
Language:English
Subjects:
Adenosine Deaminase - pharmacology
Adipose Tissue - cytology
Adipose Tissue - drug effects
Adipose Tissue - metabolism
Animals
Glucagon - pharmacology
Hydroxybutyrates - pharmacology
In Vitro Techniques
Lipolysis - drug effects
Male
Nucleoside Deaminases - pharmacology
Rats
Starvation - metabolism
Stimulation, Chemical
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Summary:1. Adipocytes isolated from epididymal adipose tissue of fed or 24 h-starved rats were incubated with a range of glucagon concentrations in the presence and absence of adenosine deaminase (4 munits/ml). 2. With adenosine deaminase present, the lipolytic response to low concentrations of glucagon (1-6 ng/ml) was considerably enhanced in cells from starved rats. 3. The effect of adenosine deaminase on basal lipolysis was altered after starvation. 4. D-3-Hydroxybutyrate (5 mM) decreased the sensitivity of lipolysis to glucagon. 5. The possible involvement of glucagon-stimulated lipolysis in the regulation of ketogenesis is briefly discussed.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj1880757