Inhibition of bone resorption in culture by inhibitors of thiol proteinases

Leupeptin, antipain, tosyl-lysylchloromethane (Tos-Lys-CH2Cl) and benzyloxy-carbonylphenylalanylalanyldiazomethane (Z-Phe-Ala-CHN2) inhibit reversibly the resorption induced by parathyroid hormone or heparin in cultured mouse bones. Leupeptin and antipain do not affect collagenase production and act...

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Bibliographic Details
Published in:Biochemical journal 1980-10, Vol.192 (1), p.365-368
Main Authors: Delaissé, J M, Eeckhout, Y, Vaes, G
Format: Article
Language:English
Subjects:
Animals
Bone and Bones - enzymology
Bone Resorption - drug effects
Cells, Cultured
Cysteine Endopeptidases
Glucuronidase - metabolism
Heparin - pharmacology
Mice
Microbial Collagenase - metabolism
Parathyroid Hormone - pharmacology
Protease Inhibitors
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Summary:Leupeptin, antipain, tosyl-lysylchloromethane (Tos-Lys-CH2Cl) and benzyloxy-carbonylphenylalanylalanyldiazomethane (Z-Phe-Ala-CHN2) inhibit reversibly the resorption induced by parathyroid hormone or heparin in cultured mouse bones. Leupeptin and antipain do not affect collagenase production and activity or the enhanced secretion of beta-glucuronidase induced by the bone-resorbing agents. They might thus act by a direct (extracellular?) inhibition of lysosomal thiol proteinases.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj1920365