Transcriptome-guided breeding for Paspalum notatum: producing apomictic hybrids with enhanced omega-3 content

• Published in: Theoretical and applied genetics 2024-12, Vol.138 (1), p.2
• Main Authors: Marino, Lara, Altabe, Silvia, Colono, Carolina Marta, Podio, Maricel, Ortiz, Juan Pablo Amelio, Balaban, David, Stein, Juliana, Spoto, Nicolás, Acuña, Carlos, Siena, Lorena Adelina, Gerde, José, Albertini, Emidio, Pessino, Silvina Claudia
• Format: Article
• Language: English
• Subjects: ABC transporter; Agriculture; Animals; Apomixis; Biochemistry; Biomedical and Life Sciences; Biotechnology; Breast milk; Breeding; Diploids; Emissions; Esters; Fatty acid methyl esters; Fatty acids; Fatty Acids, Omega-3; Gas chromatography; Genotype; Genotypes; Grazing; Greenhouse gases; Hybridization, Genetic; Hybrids; Leaves; Life Sciences; Linoleic acid; Linolenic acid; Lipid metabolism; Livestock; Mass spectroscopy; Methane; Milk; Omega-3 fatty acids; Original; Original Article; Paspalum - genetics; Paspalum notatum; Plant Biochemistry; Plant Breeding; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant Leaves - chemistry; Plant Leaves - genetics; Polyploidy; Reproduction; Transcriptome; Transcriptomes; Transcriptomics
• ISSN: 0040-5752; 1432-2242; 1432-2242
• DOI: 10.1007/s00122-024-04788-6

Key message Transcriptomics- and FAME-GC-MS-assisted apomixis breeding generated Paspalum notatum hybrids with clonal reproduction and increased α-linolenic acid content, offering the potential to enhance livestock product's nutritional quality and reduce methane emissions A low omega-6:omega-3 fatty acid ratio is considered an indicator of the nutritional impact of milk fat on human health. In ruminants, major long-chain fatty acids, such as linoleic acid (18:2, omega-6) and α-linolenic acid (18:3, omega-3), originate from dietary sources and reach the milk via the bloodstream. Since forages are the primary source of long-chain fatty acids for such animals, they are potential targets for improving milk lipid composition. Moreover, a high 18:3 content in their diet is associated with reduced methane emissions during grazing. This work aimed to develop genotypes of the forage grass Paspalum notatum with high leaf 18:3 content and the ability for clonal reproduction via seeds (apomixis). We assembled diploid and polyploid Paspalum notatum leaf transcriptomes and recovered sequences of two metabolism genes associated with the establishment of lipid profiles, namely SUGAR-DEPENDENT 1 ( SDP1 ) and PEROXISOMAL ABC TRANSPORTER 1 ( PXA1 ). Primers were designed to amplify all expressed paralogs in leaves. qPCR was used to analyse SDP1 and PXA1 expression in seven divergent genotypes. Reduced levels of SDP1 and PXA1 were found in the polyploid sexual genotype Q4188. Fatty acid methyl esters/gas chromatography/mass spectrometry (FAME/GC/MS) assays confirmed an increased percentage of 18:3 in this genotype. Crosses between Q4188 and the obligate apomictic pollen donor Q4117 resulted in two apomictic F 1 hybrids (JS9 and JS71) with reduced SDP1 and PXA1 levels, increased 18:3 content, and clonal maternal reproduction. These materials could enhance milk and meat quality while reducing greenhouse gas emissions during grazing.