MiR-92a-3p Promotes Renal Injury and Fibrosis Through Facilitating M1 Macrophage Polarization via Targeting LIN28A

• Published in: Physiological research 2024-11, Vol.73 (5/2024), p.755-767
• Main Authors: Xu, M, Zeng, X, Pan, M, Chen, R, Bai, Y, He, J, Wang, C, Qi, Y, Sun, Q, An, N
• Format: Article
• Language: English
• Subjects: Apoptosis; Cell activation; Cells; Fibrosis; Flow cytometry; Inflammation; Interleukin 6; Investigations; Ischemia; Kidney diseases; Laboratory animals; Laparotomy; Lipopolysaccharides; Macrophages; MicroRNAs; Nitric-oxide synthase; Plasmids; Polarization; Tumor necrosis factor-α
• ISSN: 0862-8408; 1802-9973
• DOI: 10.33549/physiolres.935305

Infiltrated and activated M1 macrophages play a role in kidney injury and fibrosis during chronic kidney disease (CKD) progression. However, the specific ways that M1 macrophage polarization contributes to renal fibrosis are not fully understood. The study seeks to investigate how miR-92a-3p regulates M1 macrophage polarization and its connection to renal fibrosis in the development of CKD. Our results revealed that miR-92a-3p overexpression increased M1-macrophage activation, iNOS, IL-6, and TNF-α expression in RAW264.7 upon LPS stimulation. LIN28A overexpression reversed these effects. Moreover, miR-92a-3p overexpression in RAW264.7 exacerbated NRK-52E cell apoptosis induced by LPS, but LIN28A overexpression counteracted this effect. MiR-92a-3p knockout in unilateral ureteral obstruction (UUO) C57BL/6 mice led to reduced renal infiltration and fibrosis, accompanied by decreased iNOS, α-SMA, IL-6, TNF-α, and increased LIN28A. In summary, our findings suggest that miR-92a-3p may play a role in promoting renal injury and fibrosis both in vitro and in vivo. This effect is potentially achieved by facilitating M1 macrophage polarization through the targeting of LIN28A.