Yield of Targeted Polymerase Chain Reaction in Probable Early-Onset Sepsis: A Prospective Cohort Study in Term and Near-Term Neonates With Negative Blood Culture Results

Discriminating noninfected from infected neonatal cases remains challenging, and subsequently many neonates are treated with antibiotics in the first week of life. We aimed to study the additional value of a targeted polymerase chain reaction (PCR) for group B streptococcus (GBS) and on leftover blo...

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Published in:Open forum infectious diseases 2024-12, Vol.11 (12), p.ofae681
Main Authors: Keij, Fleur M, Klaassen, Corné H W, Kornelisse, René F, van Westreenen, Mireille, Tramper-Stranders, Gerdien A
Format: Article
Language:English
Subjects:
Infectious Diseases in Special Populations
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Summary:Discriminating noninfected from infected neonatal cases remains challenging, and subsequently many neonates are treated with antibiotics in the first week of life. We aimed to study the additional value of a targeted polymerase chain reaction (PCR) for group B streptococcus (GBS) and on leftover blood culture media from term and near-term neonates with probable early-onset sepsis (EOS). Leftover blood culture material from neonates participating in the RAIN study was stored after 5 days of incubation. The RAIN study evaluated intravenous-oral antibiotic switch in probable bacterial infection, defined as risk factors and/or clinical signs and elevated inflammatory parameters but negative blood culture results. We applied 2 targeted PCRs for GBS and , the main pathogens in EOS, and analyzed the samples batchwise in triplicate for each PCR. PCR was performed in triplicate on blood culture media from 284 neonates. In 23 neonates, the PCR result was positive (3 cycle threshold values
ISSN:2328-8957
2328-8957
DOI:10.1093/ofid/ofae681