The composition and the structure of bacterioferritin of Escherichia coli

Bacterioferritin isolated from Escherichia coli is of two kinds: a protein containing a polynuclear iron compound, the bacterioferritin proper and a protein free of the polynuclear iron compound, the apo-bacterioferritin. Bacterioferritin of both kinds is characterized by absorption maxima at 417,53...

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Bibliographic Details
Published in:Biochemical journal 1981-07, Vol.197 (1), p.171-175
Main Authors: Yariv, J, Kalb, A J, Sperling, R, Bauminger, E R, Cohen, S G, Ofer, S
Format: Article
Language:English
Subjects:
Apoproteins - metabolism
Bacterial Proteins
Chemical Phenomena
Chemistry
Cytochrome b Group - metabolism
Escherichia coli - analysis
Ferritins - metabolism
Microscopy, Electron
Spectrophotometry
Spectrophotometry, Ultraviolet
X-Ray Diffraction
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Summary:Bacterioferritin isolated from Escherichia coli is of two kinds: a protein containing a polynuclear iron compound, the bacterioferritin proper and a protein free of the polynuclear iron compound, the apo-bacterioferritin. Bacterioferritin of both kinds is characterized by absorption maxima at 417,530 and 560 nm, contributed by protohaem IX. Single crystals of bacterioferritin of the space group I432 suggest that the molecule is made up of 24 identical subunits related by a cubic point symmetry. The molecular weight of the protein subunit, as determined by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis, is 15000. In the electron microscope the bacterioferritin molecule appears to be a sphere of 9.5 nm (95 A) diameter composed of a negatively staining outer shell and an inner electron-dense core of 6 nm (60 A) diameter.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj1970171