Re-activation by glutamate or aspartate of amino-oxyacetate-inhibited aspartate aminotransferase in vitro and in isolated hepatocytes

Experiments with isolated rat hepatocytes and with cell extracts indicate, in contrast with previous reports, that pyruvate does not block or reverse the inhibition of aspartate aminotransferase (EC 2.6.1.1) by amino-oxyacetate. That inhibition, however, is partially overcome by glutamate or asparta...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical journal 1981-07, Vol.198 (1), p.219-223
Main Authors: Cornell, N W, Crow, K E, Whitefoot, R P
Format: Article
Language:English
Subjects:
Acetates - pharmacology
Aminooxyacetic Acid - pharmacology
Animals
Aspartate Aminotransferases - antagonists & inhibitors
Aspartic Acid - metabolism
Aspartic Acid - pharmacology
Enzyme Activation - drug effects
Glutamates - metabolism
Glutamates - pharmacology
In Vitro Techniques
Liver - cytology
Liver - drug effects
Liver - enzymology
Male
Metabolism, Regulation and Control Processes
Rats
Rats, Inbred Strains
Urea - biosynthesis
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Experiments with isolated rat hepatocytes and with cell extracts indicate, in contrast with previous reports, that pyruvate does not block or reverse the inhibition of aspartate aminotransferase (EC 2.6.1.1) by amino-oxyacetate. That inhibition, however, is partially overcome by glutamate or aspartate either in cell extracts or in whole cells incubated with substrate combinations that cause accumulation of those amino acids.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj1980219