High Expression of RAB32 Predicts Adverse Outcomes: A Potential Therapeutic Target for Glioblastoma

• Published in: Journal of Cancer 2024-10, Vol.15 (20), p.6710-6723
• Main Authors: Huang, Liji, Chi, Yue, Su, Xiangyue, Zhang, Hongyu, Cao, Yanfei, Wang, Xindi, Zhang, Sinan, Jiang, Xudong, Zhang, Lina
• Format: Article
• Language: English
• Subjects: Research Paper
• ISSN: 1837-9664; 1837-9664
• DOI: 10.7150/jca.96162

RAB32 is a potential prognostic marker that is overexpressed in a variety of cancers. The purpose of this study was to investigate the expression and function of RAB32 in glioblastoma (GBM). The RAB32 expression data were obtained by accessing the TCGA, CGGA and GEPIA databases and were verified by western blot and immunohistochemistry. The prognostic value of RAB32 methylation was carefully examined using cBioPortal and MethSurv. GSEA was used to analyze cancer-related signaling pathways that may be activated by high RAB32 expression. The correlation between RAB32 and GBM infiltration was studied by accessing the TISIDB database. The effects of RAB32 on the proliferation, migration and invasion of GBM cells were predicted by colony formation assay, CCK-8 assay and Transwell assay. In this study, RAB32 expression was upregulated in GBM compared to normal brain tissue. Survival analysis showed that high expression of RAB32 was an independent risk factor for overall survival in glioma patients. RAB32 methylation was negatively correlated with RAB32 expression, and the overall survival rate of patients with RAB32 hypomethylation was lower than that of patients with RAB32 hypermethylation. Through functional enrichment analysis, we found that RAB32 overexpression significantly activated multiple signaling pathways. The immunoassay results showed that RAB32 expression was correlated with immune infiltration of the tumor microenvironment. Knocking down the expression of the RAB32 gene significantly inhibited the proliferation, migration and invasion of glioma cells. Our results show that RAB32 is a key factor affecting the prognosis of patients with GBM, and its targeting may provide a new treatment for patients with GBM.