Partial diploids of Escherichia coli carrying normal and mutant alleles affecting oxidative phosphorylation

A plasmid was isolated which included the region of the Escherichia coli chromosome carrying the known genes concerned with oxidative phosphorylation (unc genes). This plasmid was used to prepare partial diploids carrying normal unc alleles on the episome and one of the three mutant alleles (unc A40...

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Bibliographic Details
Published in:Biochemical journal 1977-03, Vol.162 (3), p.665-670
Main Authors: Gibson, F, Cox, G B, Downie, J A, Radik, J
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - metabolism
Alleles
Diploidy
Escherichia coli - growth & development
Escherichia coli - metabolism
Fluorescence
Genetic Techniques
Mutation
NADH, NADPH Oxidoreductases - metabolism
Oxidative Phosphorylation
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Summary:A plasmid was isolated which included the region of the Escherichia coli chromosome carrying the known genes concerned with oxidative phosphorylation (unc genes). This plasmid was used to prepare partial diploids carrying normal unc alleles on the episome and one of the three mutant alleles (unc A401, uncB402 or unc-405) on the chromosome. These strains were compared with segregants from which the plasmid had been lost. Dominance of either normal ormutant unc alleles was determined by growth on succinate, growth yields on glucose, Mg-ATPase (Mg2+-stimulated adenosine triphosphatase) activity, atebrin-fluorescence quenching, ATP-dependent transhydrogenase activity and oxidative phosphorylation. In all the above tests, dominance of the normal allele was observed. However, in membranes from the diploid strains which carried a normal allele and either of the mutant alleles affecting Mg-ATPase activity (uncA401 or unc-405), the energy-linked functions were only partially restored.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj1620665