Role of Lysocardiolipin Acyltransferase in Cigarette Smoke-Induced Lung Epithelial Cell Mitochondrial ROS, Mitochondrial Dynamics, and Apoptosis

• Published in: Cell biochemistry and biophysics 2022-03, Vol.80 (1), p.203-216
• Main Authors: Bandela, Mounica, Suryadevara, Vidyani, Fu, Panfeng, Reddy, Sekhar P., Bikkavilli, Kamesh, Huang, Long Shuang, Dhavamani, Sugasini, Subbaiah, Papasani V., Singla, Sunit, Dudek, Steven M., Ware, Lorraine B., Ramchandran, Ramaswamy, Natarajan, Viswanathan
• Format: Article
• Language: English
• Subjects: Acyltransferase; Acyltransferases - genetics; Acyltransferases - metabolism; Animal tissues; Animals; Apoptosis; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Biotechnology; Cardiolipin; Cell Biology; Chronic obstructive pulmonary disease; Cigarette smoke; Cigarettes; Composition; Epithelial cells; Epithelial Cells - metabolism; Epithelium; Fatty acid composition; Fatty acids; Fission; Gene expression; Life Sciences; Lung - metabolism; Lungs; Lysocardiolipin; Mice; Mitochondria; Mitochondria - metabolism; Mitochondrial Dynamics; mRNA; Original Paper; Oxidative stress; Pharmacology/Toxicology; Pulmonary Disease, Chronic Obstructive - metabolism; Reactive oxygen species; Reactive Oxygen Species - metabolism; Smoke; Smoking; Smoking - adverse effects; Tobacco smoke
• ISSN: 1085-9195; 1559-0283; 1559-0283
• DOI: 10.1007/s12013-021-01043-3

Cigarette smoke is the primary cause of Chronic Obstructive Pulmonary Disorder (COPD). Cigarette smoke extract (CSE)-induced oxidative damage of the lungs results in mitochondrial dysfunction and apoptosis of epithelium. Mitochondrial cardiolipin (CL) present in the inner mitochondrial membrane plays an important role in mitochondrial function, wherein its fatty acid composition is regulated by lysocardiolipin acyltransferase (LYCAT). In this study, we investigated the role of LYCAT expression and activity in mitochondrial oxidative stress, mitochondrial dynamics, and lung epithelial cell apoptosis. LYCAT expression was increased in human lung specimens from smokers, and cigarette smoke-exposed-mouse lung tissues. Cigarette smoke extract (CSE) increased LYCAT mRNA levels and protein expression, modulated cardiolipin fatty acid composition, and enhanced mitochondrial fission in the bronchial epithelial cell line, BEAS-2B in vitro. Inhibition of LYCAT activity with a peptide mimetic, attenuated CSE-mediated mitochondrial (mt) reactive oxygen species (ROS), mitochondrial fragmentation, and apoptosis, while MitoTEMPO attenuated CSE-induced MitoROS, mitochondrial fission and apoptosis of BEAS-2B cells. Collectively, these findings suggest that increased LYCAT expression promotes MitoROS, mitochondrial dynamics and apoptosis of lung epithelial cells. Given the key role of LYCAT in mitochondrial cardiolipin remodeling and function, strategies aimed at inhibiting LYCAT activity and ROS may offer an innovative approach to minimize lung inflammation caused by cigarette smoke.