A simple and rapid method for the preparation of adenosine triphosphatase from submitochondrial particles

An almost pure form of the bovine heart mitochondrial adenosine triphosphatase (ATPase) is released from the membrane by shaking submitochondrial particles with chloroform. Analyses on polyacrylamide gels and by electron microscopy, and also sensitivity to inhibitors, show that the chloroform-releas...

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Bibliographic Details
Published in:Biochemical journal 1975-06, Vol.148 (3), p.533-537
Main Authors: Beechey, R B, Hubbard, S A, Linnett, P E, Mitchell, A D, Munn, E A
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - antagonists & inhibitors
Adenosine Triphosphatases - isolation & purification
Animals
Aurovertins - pharmacology
Cattle
Chloroform
Dicyclohexylcarbodiimide - pharmacology
Electrophoresis, Polyacrylamide Gel
In Vitro Techniques
Methods
Microscopy, Electron
Mitochondria, Muscle - enzymology
Myocardium - enzymology
Subcellular Fractions - analysis
Ultracentrifugation
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Description
Summary:An almost pure form of the bovine heart mitochondrial adenosine triphosphatase (ATPase) is released from the membrane by shaking submitochondrial particles with chloroform. Analyses on polyacrylamide gels and by electron microscopy, and also sensitivity to inhibitors, show that the chloroform-released enzyme is similar to other ATPase preparations from bovine heart mitochondria.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj1480533