Thermolability of 28S ribosomal ribonucleic acid from the liver of Crotalus durissus terrificus (Ophidia, Reptilia)

Instability of 28S rRNA of Crotalus durissus terrificus liver was observed during hotphenol extraction: purified 28S rRNA is converted into an 18S RNA component by heat treatment. It was also found that ;6S' and ;8S' low-molecular-weight RNA species were released during the thermal convers...

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Bibliographic Details
Published in:Biochemical journal 1973-09, Vol.135 (1), p.73-79
Main Authors: Giorgini, J F, De Lucca, F L
Format: Article
Language:English
Subjects:
Animals
Centrifugation, Density Gradient
Electrophoresis, Polyacrylamide Gel
Hot Temperature
Liver - analysis
Molecular Weight
Nucleic Acid Denaturation
Nucleic Acids
Phenols
RNA, Ribosomal
Snakes - metabolism
Sulfoxides
Thermodynamics
Urea
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Summary:Instability of 28S rRNA of Crotalus durissus terrificus liver was observed during hotphenol extraction: purified 28S rRNA is converted into an 18S RNA component by heat treatment. It was also found that ;6S' and ;8S' low-molecular-weight RNA species were released during the thermal conversion. This conversion and the release of the low-molecular-weight species were also induced by 8m-urea and 80% (v/v) dimethyl sulphoxide at 0 degrees C. Evidence is presented that this phenomenon is an irreversible process and results from the rupture of hydrogen bonds. The 18S RNA product was shown to be homogeneous by polyacrylamide-gel electrophoresis and by sucrose-density-gradient centrifugation. The base composition of the 18S RNA products obtained by heat, urea or dimethyl sulphoxide treatments was similar. The C+G content of the 18S RNA product was different from that of the native 18S rRNA, but similar to that of 28S rRNA.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj1350073