Evidence for the binding of the carcinogen 3-methylcholanthrene to both the purine and the pyrimidine bases of hamster fibroblast deoxyribonucleic acid

The binding of [(3)H]3-methylcholanthrene to the DNA of hamster fibroblasts was studied by using chemical methods for DNA degradation. DNA depurinated by mild acid hydrolysis released approximately half of the radioactivity at the same rate as the purine bases, but the resulting apurinic acid still...

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Bibliographic Details
Published in:Biochemical journal 1973-10, Vol.135 (2), p.375-378
Main Authors: Jones, P A, Gevers, W, Hawtrey, A O
Format: Article
Language:English
Subjects:
Animals
Binding Sites
Chromatography
Cricetinae
Culture Techniques
DNA
Fibroblasts
Hydrolysis
Methylcholanthrene
Nucleic Acids
Purine Nucleotides
Pyrimidine Nucleotides
Tritium
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Summary:The binding of [(3)H]3-methylcholanthrene to the DNA of hamster fibroblasts was studied by using chemical methods for DNA degradation. DNA depurinated by mild acid hydrolysis released approximately half of the radioactivity at the same rate as the purine bases, but the resulting apurinic acid still contained radioactive carcinogen.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj1350375