The use of sodium perchlorate in deproteinization during the preparation of nucleic acids

Sodium perchlorate in high concentrations will remove from solution the detergent sodium dodecyl sulphate and protein complexed with it. This and the failure of proteins to be precipitated by ethanol from solutions containing a high concentration of sodium perchlorate can be utilized as efficient, r...

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Bibliographic Details
Published in:Biochemical journal 1973-11, Vol.135 (3), p.559-561
Main Author: Wilcockson, J
Format: Article
Language:English
Subjects:
Adenine
Chemical Precipitation
DNA - isolation & purification
Ethanol
Methods
Nucleic Acids
Perchlorates
Proteins
RNA - isolation & purification
Sodium Dodecyl Sulfate
Tritium
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Summary:Sodium perchlorate in high concentrations will remove from solution the detergent sodium dodecyl sulphate and protein complexed with it. This and the failure of proteins to be precipitated by ethanol from solutions containing a high concentration of sodium perchlorate can be utilized as efficient, rapid and simple deproteinization procedures during the preparation of nucleic acids.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj1350559