Respiration-driven proton translocation in Escherichia coli

Measurements were made of the stoicheiometry of respiration-driven proton translocation coupled to the oxidation of NAD(P)-linked or flavin-linked substrates in intact cells of Escherichia coli. Observed stoicheiometries (-->H(+)/O quotient; Mitchell, 1966) were approx. 4 with l-malate as substra...

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Bibliographic Details
Published in:Biochemical journal 1973-09, Vol.136 (1), p.217-220
Main Authors: Lawford, H G, Haddock, B A
Format: Article
Language:English
Subjects:
Aerobiosis
Bioenergetics
Electron Transport
Energy Metabolism
Escherichia coli - growth & development
Escherichia coli - metabolism
Flavins - metabolism
Glycerol - metabolism
Lactates - metabolism
Malates - metabolism
NAD - metabolism
NADP - metabolism
Oxidation-Reduction
Oxygen Consumption
Protons
Succinates - metabolism
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Summary:Measurements were made of the stoicheiometry of respiration-driven proton translocation coupled to the oxidation of NAD(P)-linked or flavin-linked substrates in intact cells of Escherichia coli. Observed stoicheiometries (-->H(+)/O quotient; Mitchell, 1966) were approx. 4 with l-malate as substrate and approx. 2 for succinate, d-lactate and glycerol oxidation. It is concluded that the potential number of equivalent energy-conservation sites associated with the respiratory chain is 2 in aerobically grown cells of E. coli harvested during the exponential phase of growth.
ISSN:0264-6021
0306-3283
1470-8728
DOI:10.1042/bj1360217