Development of the SupersonicIEF Method for High‐Throughput Charge Variant Analysis

ABSTRACT The analysis of biopharmaceuticals for charge variants occurs from early‐stage samples through formulation and process‐development optimization. Higher throughput methods allow increased analysis of these samples to facilitate greater understanding of the samples and to better optimize thei...

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Bibliographic Details
Published in:Electrophoresis 2024-11, Vol.45 (21-22), p.1968-1975
Main Authors: McElroy, Will, Heger, Christopher D.
Format: Article
Language:English
Subjects:
charge heterogeneity
Electrophoresis, Capillary - methods
fusion proteins
High-Throughput Screening Assays - methods
imaged capillary isoelectric focusing
Isoelectric focusing
Isoelectric Focusing - methods
Liquid Phase Separations
monoclonal antibodies
Optimization
platform method
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Summary:ABSTRACT The analysis of biopharmaceuticals for charge variants occurs from early‐stage samples through formulation and process‐development optimization. Higher throughput methods allow increased analysis of these samples to facilitate greater understanding of the samples and to better optimize their production and formulation. To enable higher throughput charge variant analysis, a new, rapid platform imaged capillary isoelectric focusing (icIEF) method was optimized to be two to three times faster than standard methods.
ISSN:0173-0835
1522-2683
1522-2683
DOI:10.1002/elps.202400117