Evidence from molecular cloning that SPARC, a major product of mouse embryo parietal endoderm, is related to an endothelial cell ‘culture shock’ glycoprotein of Mr 43,000

• Published in: The EMBO journal 1986-07, Vol.5 (7), p.1465-1472
• Main Authors: Mason, I.J., Taylor, A., Williams, J.G., Sage, H., Hogan, B.L.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Biotechnology; Cattle; Cells, Cultured; Cloning, Molecular; DNA - isolation & purification; Embryo, Mammalian; Endoderm - metabolism; Endothelium - metabolism; Fundamental and applied biological sciences. Psychology; Genetic engineering; Genetic technics; Glycoproteins - genetics; Glycoproteins - isolation & purification; Methods. Procedures. Technologies; Mice; Molecular cloning; Molecular Sequence Data; Molecular Weight; Osteonectin; Sequence Homology, Nucleic Acid; Species Specificity; Transcription, Genetic; Tumor Suppressor Proteins - genetics; Tumor Suppressor Proteins - isolation & purification
• ISSN: 0261-4189; 1460-2075
• DOI: 10.1002/j.1460-2075.1986.tb04383.x

We describe the molecular cloning and characterization of a secreted, acidic, cysteine‐rich glycoprotein (SPARC) of apparent Mr 43,000 which is a major product of mouse embryo parietal endoderm. These cells are specialized for the synthesis of a rapidly expanding basement membrane, but SPARC is not itself an integral matrix component. We show that SPARC is related structurally and antigenically to an Mr 43,000 glycoprotein secreted in large amounts by bovine aortic endothelial cells as part of a ‘culture shock’ response to in vitro conditions promoting their proliferation and migration.