The gene III product (P15) of cauliflower mosaic virus is a DNA‐binding protein while an immunologically related P11 polypeptide is associated with virions

Using electron microscopic immunocytochemical staining we demonstrate that the product of gene III of cauliflower mosaic virus (CaMV) is associated with viral particles. Furthermore, a fusion protein, expressed in bacteria, consisting of the N‐terminus of β‐galactosidase and the complete gene III pr...

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Bibliographic Details
Published in:The EMBO journal 1986-10, Vol.5 (10), p.2433-2438
Main Authors: Giband, M., Mesnard, J.M., Lebeurier, G.
Format: Article
Language:English
Subjects:
Biological and medical sciences
cauliflower mosaic virus
DNA
DNA‐binding protein
electron microscopy
Fundamental and applied biological sciences. Psychology
fusion protein
gene III product
gene products
genes
Genetics
immunochemistry
immunocytochemical staining
Life Sciences
Microbiology
Microbiology and Parasitology
staining
Virology
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Summary:Using electron microscopic immunocytochemical staining we demonstrate that the product of gene III of cauliflower mosaic virus (CaMV) is associated with viral particles. Furthermore, a fusion protein, expressed in bacteria, consisting of the N‐terminus of β‐galactosidase and the complete gene III protein of CaMV showed DNA‐binding activity. From these two results, we discuss the possible function of this viral polypeptide.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1986.tb04518.x