Probing the topology of a bacterial membrane protein by genetic insertion of a foreign epitope; expression at the cell surface

The LamB protein is a trimeric integral outer membrane protein from Escherichia coli K12 which functions as a pore for maltodextrins and a receptor for bacteriophages. When inserted into two selected sites of LamB, a foreign antigen, the C3 epitope from poliovirus, was exposed at the cell surface wi...

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Bibliographic Details
Published in:The EMBO journal 1986-11, Vol.5 (11), p.3029-3037
Main Authors: Charbit, A., Boulain, J.C., Ryter, A., Hofnung, M.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
antigens
Bacterial Outer Membrane Proteins - genetics
Bacterial Outer Membrane Proteins - physiology
Bacteriology
Base Sequence
Biogenesis of cell structures, supramolecular organization
Biological and medical sciences
Cell Membrane - physiology
Cell Membrane - ultrastructure
DNA Transposable Elements
Epitopes - analysis
Escherichia coli
Escherichia coli - genetics
Escherichia coli - physiology
Escherichia coli - ultrastructure
Fundamental and applied biological sciences. Psychology
Immune Sera
influenza virus
LamB protein
membrane proteins
Microbiology
Microscopy, Electron
Models, Molecular
Protein Conformation
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Summary:The LamB protein is a trimeric integral outer membrane protein from Escherichia coli K12 which functions as a pore for maltodextrins and a receptor for bacteriophages. When inserted into two selected sites of LamB, a foreign antigen, the C3 epitope from poliovirus, was exposed at the cell surface with its normal antigenic properties. Since these genetic insertions did not affect in any essential way the routing, activity and folding of the LamB protein, we conclude that the two corresponding LamB sites are at the cell surface as predicted by our recent model. We discuss the implications of our results for the study of protein topology with a single epitope and the direct cloning and cell surface expression of epitopes of interest as well as the development of live vaccines or diagnostic tests.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1986.tb04602.x