Molecular Profiling of A549 Cell-Derived Exosomes: Proteomic, miRNA, and Interactome Analysis for Identifying Potential Key Regulators in Lung Cancer

Exosomes are small extracellular vesicles released by all cells that play a crucial role in cell-to-cell communication. Exosomes derived from cancer cells carry tumorigenic properties, transmitting these signals to surrounding cells within the tumor microenvironment or to distant sites. However, the...

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Bibliographic Details
Published in:Cancers 2024-12, Vol.16 (24), p.4123
Main Authors: Giannopoulos-Dimitriou, Alexandros, Saiti, Aikaterini, Malousi, Andigoni, Anagnostopoulos, Athanasios K, Vatsellas, Giannis, Al-Maghrabi, Passant M, Müllertz, Anette, Fatouros, Dimitrios G, Vizirianakis, Ioannis S
Format: Article
Language:English
Subjects:
Adenocarcinoma
Angiogenesis
Bioinformatics
Biomarkers
Cell culture
Cell growth
Cell interactions
Development and progression
Exosomes
Fibroblasts
Lasers
Lung cancer
MicroRNA
MicroRNAs
miRNA
Morphology
Penicillin
Proteins
Proteomics
Tumor microenvironment
Tumors
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Summary:Exosomes are small extracellular vesicles released by all cells that play a crucial role in cell-to-cell communication. Exosomes derived from cancer cells carry tumorigenic properties, transmitting these signals to surrounding cells within the tumor microenvironment or to distant sites. However, the complexity of exosome cargo remains poorly understood. In this study, we isolated exosomes from human lung adenocarcinoma A549 cell cultures, characterized their physicochemical and morphological properties, and analyzed their proteomic and miRNA cargo through high-throughput profiling and bioinformatics. Key molecules within the exosomal cargo were identified, and network analysis revealed their potential effects on the physiology of potential recipient cells. Furthermore, we compared the miRNA profiles of malignant A549 exosomes with those from normal lung fibroblast MRC-5 cells, identifying tumor-associated miRNAs for potential biomarker exploration in clinical samples. This study advances our understanding of exosomal molecular components and their interactions, opening avenues for further research into their roles in cancer.
ISSN:2072-6694
2072-6694
DOI:10.3390/cancers16244123