Effect of nitrate on the synthesis and decay of nitrate reductase of Neurospora

1. A method was developed to examine the turnover of nitrate reductase by the use of tungstate. 2. Evidence is presented which suggests that the disappearance of nitrate reductase activity from Neurospora mycelia exposed to non-inducing conditions is due to the disappearance of the enzyme protein(s)...

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Bibliographic Details
Published in:Biochemical journal 1974-06, Vol.140 (3), p.395-403
Main Authors: Sorger, G J, Debanne, M T, Davies, J
Format: Article
Language:English
Subjects:
Animals
Antibodies, Fungal
Benzyl Compounds
Cellular Interactions and Control Processes
Cycloheximide - metabolism
Enzyme Induction
NADP
Neurospora - drug effects
Neurospora - metabolism
Nitrate Reductases - biosynthesis
Nitrate Reductases - metabolism
Nitrates - metabolism
Nitrates - pharmacology
Operon
Rabbits - immunology
Tungsten - pharmacology
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Summary:1. A method was developed to examine the turnover of nitrate reductase by the use of tungstate. 2. Evidence is presented which suggests that the disappearance of nitrate reductase activity from Neurospora mycelia exposed to non-inducing conditions is due to the disappearance of the enzyme protein(s) from the mycelia, and not merely due to the disappearance of its (their) catalytic power. 3. The presence of NO(3) (-) in the culture medium slows down the rate of degradation of nitrate reductase in Neurospora in vivo.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj1400395