Mammalian chromatin substructure studies with the calcium-magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis

The basic regularity of chromatin substructure that has been reported in rat liver chromatin (Hewish & Burgoyne, 1973b) was also detected in mouse chromatin. The regular series of DNA fragments produced by the action of Ca-Mg endonuclease on rat chromatin were studied further. The smallest singl...

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Bibliographic Details
Published in:Biochemical journal 1974-10, Vol.143 (1), p.67-72
Main Authors: Burgoyne, L A, Hewish, D R, Mobbs, J
Format: Article
Language:English
Subjects:
Animals
Calcium
Cell Nucleus - analysis
Centrifugation, Density Gradient
Chromatin - analysis
DNA - analysis
DNA - isolation & purification
Electrophoresis, Polyacrylamide Gel - methods
Endonucleases
Liver - analysis
Magnesium
Methods
Mice
Molecular Weight
Nucleic Acid Denaturation
Nucleic Acids
Rats
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Summary:The basic regularity of chromatin substructure that has been reported in rat liver chromatin (Hewish & Burgoyne, 1973b) was also detected in mouse chromatin. The regular series of DNA fragments produced by the action of Ca-Mg endonuclease on rat chromatin were studied further. The smallest single-stranded class has a molecular weight of approx. 45000-63000 and the smallest double-stranded class has a molecular weight of approx. 120000-150000. Studies of the substructure of the DNA fragments produced by the Ca-Mg endonuclease have shown that the regular series of double-stranded fragments have regular series of single-stranded fragments within them. It was concluded that the regular series of double-stranded fragments was probably a consequence of the regular series of single-stranded fragments. Digestion time-courses are presented for mouse and rat nuclear DNA.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj1430067