The role of the nuclear pore complex in adenovirus DNA entry

Adenovirus targets its genome to the cell nucleus by a multistep process involving endocytosis, membrane penetration and cytoplasmic transport, and finally imports its DNA into the nucleus. Using an immunochemical and biochemical approach combined with inhibitors of nuclear import, we demonstrate th...

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Bibliographic Details
Published in:The EMBO journal 1997-10, Vol.16 (19), p.5998-6007
Main Authors: Greber, Urs F., Suomalainen, Maarit, Stidwill, Robert P., Boucke, Karin, Ebersold, Melanie W., Helenius, Ari
Format: Article
Language:English
Subjects:
Adenoviridae - genetics
Adenoviridae - physiology
adenovirus
Antibodies
Biological Transport - drug effects
calcium
Calcium - metabolism
Capsid - metabolism
Cell Nucleus - metabolism
Cell Nucleus - virology
DNA
DNA, Viral - metabolism
HeLa Cells
Humans
In Situ Hybridization, Fluorescence
Nuclear Envelope - metabolism
Nuclear Envelope - virology
nuclear import
nuclear pore complex
Viral Structural Proteins - metabolism
Wheat Germ Agglutinins - pharmacology
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Summary:Adenovirus targets its genome to the cell nucleus by a multistep process involving endocytosis, membrane penetration and cytoplasmic transport, and finally imports its DNA into the nucleus. Using an immunochemical and biochemical approach combined with inhibitors of nuclear import, we demonstrate that incoming viral DNA and DNA‐associated protein VII enter the nucleus via nuclear pore complexes (NPCs). Depletion of calcium from nuclear envelope and endoplasmic reticulum cisternae by ionophores or thapsigargin blocked DNA and protein VII import into the nucleus, but had no effect on virus targeting to NPCs. Calcium‐depleted cells were capable of disassembling incoming virus. In contrast, inhibitors of cytosolic O ‐linked glycoproteins of the NPC blocked virus attachment to the nuclear envelope, capsid disassembly and also nuclear import of protein VII. The data indicate that NPCs have multiple roles in adenovirus entry into cells: they contain a virus‐binding and/or dissociation activity and provide a gateway for the incoming DNA genome into the nucleus.
ISSN:0261-4189
1460-2075
DOI:10.1093/emboj/16.19.5998