Budding yeast RSI1/APC2, a novel gene necessary for initiation of anaphase, encodes an APC subunit

• Published in: The EMBO journal 1998-01, Vol.17 (2), p.498-506
• Main Authors: Kramer, K.K, Fesquet, D, Johnson, A.L, Johnston, L.H
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; amino acid sequences; Anaphase - genetics; anaphase promoting complex; Anaphase-Promoting Complex-Cyclosome; APC; Apc2 Subunit, Anaphase-Promoting Complex-Cyclosome; Apc8 Subunit, Anaphase-Promoting Complex-Cyclosome; binding proteins; cell cycle; Cell Cycle Proteins - genetics; Cyclin B; Cyclin-Dependent Kinase Inhibitor Proteins; Cyclins - deficiency; Cyclins - metabolism; Cyclins - physiology; deletions; Fungal Proteins - genetics; Fungal Proteins - metabolism; Fungal Proteins - physiology; genbank/z73299; Genes, Fungal; Genes, Lethal; initiation; lethal genes; ligases; Ligases - genetics; Molecular Sequence Data; Mutagenesis; mutants; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; nucleotide sequences; Phenotype; promoter regions; rsi1 gene; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - isolation & purification; Saccharomyces cerevisiae Proteins; Securin; SIC1; structural genes; Temperature; ubiquitin ligase; Ubiquitin-Protein Ligase Complexes; Ubiquitin-Protein Ligases; yeast
• ISSN: 0261-4189; 1460-2075; 1460-2075
• DOI: 10.1093/emboj/17.2.498

SIC1 is a non‐essential gene encoding a CDK inhibitor of Cdc28‐Clb kinase activity. Sic1p is involved in both mitotic exit and the timing of DNA synthesis. To identify other genes involved in controlling Clb‐kinase activity, we have undertaken a genetic screen for mutations which render SIC1 essential. Here we describe a gene we have identified by this means, RSI1/APC2. Temperature‐sensitive rsi1/apc2 mutants arrest in metaphase and are unable to degrade Clb2p, suggesting that Rsi1p/Apc2p is associated with the anaphase promoting complex (APC). This is an E3 ubiquitin‐ligase that controls anaphase initiation through degradation of Pds1p and mitotic exit via degradation of Clb cyclins. Indeed, the anaphase block in rsi1/apc2 temperature‐sensitive mutants is overcome by removal of PDS1, consistent with Rsi1p/Apc2p being part of the APC. In addition, like our rsi1/apc2 mutations, cdc23‐1, encoding a known APC subunit, is also lethal with sic1Δ. Thus SIC1 clearly becomes essential when APC function is compromised. Finally, we find that Rsi1p/Apc2p co‐immunoprecipitates with Cdc23p. Taken together, our results suggest that RSI1/APC2 is a subunit of APC.