Changes in lectin-induced deoxyribonucleic acid synthesis in cultures of chick-embryo fibroblasts at various stages of development

Concanavalin A and Robinia pseudoacacia lectin decreased [(3)H]thymidine incorporation into acid-insoluble material of fibroblasts cultured from 6-10-day chick embryos. In contrast, these lectins stimulated [(3)H]thymidine incorporation in cells from 16-day embryos. These effects are due to neither...

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Bibliographic Details
Published in:Biochemical journal 1975-11, Vol.152 (2), p.421-423
Main Authors: Roguet, R, Bourrillon, R
Format: Article
Language:English
Subjects:
Animals
Cell Differentiation
Cells, Cultured
Chick Embryo
Concanavalin A - pharmacology
Development
DNA - biosynthesis
Fibroblasts - drug effects
Fibroblasts - metabolism
Lectins - pharmacology
Thymidine - metabolism
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Summary:Concanavalin A and Robinia pseudoacacia lectin decreased [(3)H]thymidine incorporation into acid-insoluble material of fibroblasts cultured from 6-10-day chick embryos. In contrast, these lectins stimulated [(3)H]thymidine incorporation in cells from 16-day embryos. These effects are due to neither [(3)H]thymidine permeability modification nor toxicity of the lectins. The specificity of lectin action was proved by blocking experiments with alpha-methyl mannopyranoside and with anti-(Robinia lectin) serum.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj1520421