Antiviral Activity of Ecklonia cava Extracts and Dieckol Against Zika Virus

Ecklonia cava and its major compound dieckol, both natural marine products, possess antioxidant, anti-inflammatory, and metabolic-regulating effects. Zika virus (ZIKV), an arbovirus from the Flaviviridae family, is transmitted by mosquitoes and causes serious illnesses in humans. This study aimed to...

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Published in:International journal of molecular sciences 2024-12, Vol.25 (24), p.13694
Main Authors: Kim, Eun-A, Kang, Nalae, Heo, Jun-Ho, Park, Areumi, Heo, Seong-Yeong, Kim, Hyun-Soo, Heo, Soo-Jin
Format: Article
Language:English
Subjects:
Antiviral agents
Antiviral drugs
Biological response modifiers
Cytokines
Dengue fever
Disease transmission
Force and energy
Guillain-Barre syndrome
Immune system
Infections
Medical research
Medicine, Experimental
Natural products
Polyphenols
Protein binding
Proteins
Public health
RNA
RNA polymerase
Tumor necrosis factor-TNF
Viral infections
Zika virus
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Summary:Ecklonia cava and its major compound dieckol, both natural marine products, possess antioxidant, anti-inflammatory, and metabolic-regulating effects. Zika virus (ZIKV), an arbovirus from the Flaviviridae family, is transmitted by mosquitoes and causes serious illnesses in humans. This study aimed to evaluate the anti-ZIKV potential of Ecklonia cava and dieckol. The antiviral activity of Ecklonia cava extract (ECE), prepared with 80% ethanol, was assessed in ZIKV-infected Vero E6 cells through MTT assay, plaque assay, and quantitative polymerase chain reaction (qPCR), demonstrating no cytotoxicity and a significant reduction in viral titers and ZIKV mRNA levels. In addition, ECE decreased the expression of tumor necrosis factor-α and interferon-induced protein with tetratricopeptide repeats in the ZIKV-infected cells. Dieckol, the primary active compound in ECE, exhibited potent anti-ZIKV activity, with a half maximal inhibitory concentration (IC50), value of 4.8 µM. In silico molecular docking analysis revealed that dieckol forms stable complexes with key ZIKV proteins, including the envelope, NS2B/NS3, and RNA-dependent RNA polymerase (RdRp) protein, exhibiting high binding energies of −438.09 kcal/mol, −1040.51 kcal/mol, and −1043.40 kcal/mol, respectively. Overall, our findings suggest that ECE and dieckol are promising candidates for the development of anti-ZIKV agents.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms252413694