Synthesis of ribonucleic acid in the venom gland of Crotalus durissus terrificus (Ophidia, Reptilia) after manual extraction of the venom

• Published in: Biochemical journal 1972-11, Vol.130 (2), p.335-342
• Main Authors: De Lucca, F. L., Imaizumi, M. T.
• Format: Article
• Language: English
• ISSN: 0306-3283; 0264-6021; 1470-8728
• DOI: 10.1042/bj1300335

1. The incorporation of [5-3H]uridine into RNA of the venom gland of Crotalus durissus terrificus was studied after manual extraction (‘milking’) of the venom. The labelled precursor was injected immediately after milking. 2. The RNA was extracted 1, 2, 4, 6 and 8h after injection of the label and analysed by sucrose-density-gradient centrifugation. 3. The sedimentation analysis showed that 18S rRNA synthesis is higher than 28S rRNA at all time-intervals. The specific radioactivities of both ribosomal components did not reach a plateau even at 8h after injection. 4. An RNA fraction was detected sedimenting between 18S rRNA and 4S tRNA and was called the 10–14S fraction. The specific radioactivity was always higher than that of both classes of rRNA and reached the maximum value at 6h of labelling. 5. The incorporation of the precursor was also studied by radioautography, which helped to elucidate the intracellular origin of the RNA analysed by sucrose-density-gradient centrifugation.