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Luminal Ca2+ promoting spontaneous Ca2+ release from inositol trisphosphate-sensitive stores in rat hepatocytes
1. Spontaneous Ca2+ release from the inositol 1,4,5-trisphosphate (InsP3)-sensitive stores in permeabilized hepatocytes was monitored using Fluo-3 to measure the free [Ca2+] of the medium bathing the cells. 2. Permeabilized cells rapidly sequestered Ca2+, reducing the [Ca2+] to 103 +/- 5 nM. Under c...
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| Published in: | The Journal of physiology 1992-09, Vol.455 (1), p.623-640 |
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| container_title | The Journal of physiology |
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| creator | Missiaen, L Taylor, C W Berridge, M J |
| description | 1. Spontaneous Ca2+ release from the inositol 1,4,5-trisphosphate (InsP3)-sensitive stores in permeabilized hepatocytes was
monitored using Fluo-3 to measure the free [Ca2+] of the medium bathing the cells. 2. Permeabilized cells rapidly sequestered
Ca2+, reducing the [Ca2+] to 103 +/- 5 nM. Under conditions that depended critically upon cell density and the amount of Ca2+
in the medium, this was followed by a slow increase in [Ca2+] culminating in a substantial Ca2+ spike representing synchronous
discharge from the InsP3-sensitive stores. 3. During the latency preceding the Ca2+ spike, the stores increased their sensitivity
to InsP3. This sensitization seemed to be an all-or-none phenomenon. 4. Oxidized glutathione and thimerosal promoted the spontaneous
release by sensitizing the InsP3 receptor. 5. An increase in the [Ca2+] within the stores was required for both the increased
sensitivity to InsP3 and the subsequent spike. 6. Caffeine (6 mM) antagonized the effect of very low InsP3 concentrations
and abolished the Ca2+ spike, without itself releasing Ca2+. 7. Our results suggesting that luminal Ca2+ may sensitive InsP3-sensitive
stores leading to spontaneous Ca2+ mobilization will be discussed in the light of a modified version of the two-pool model
for explaining cytosolic Ca2+ oscillations. |
| doi_str_mv | 10.1113/jphysiol.1992.sp019319 |
| format | article |
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monitored using Fluo-3 to measure the free [Ca2+] of the medium bathing the cells. 2. Permeabilized cells rapidly sequestered
Ca2+, reducing the [Ca2+] to 103 +/- 5 nM. Under conditions that depended critically upon cell density and the amount of Ca2+
in the medium, this was followed by a slow increase in [Ca2+] culminating in a substantial Ca2+ spike representing synchronous
discharge from the InsP3-sensitive stores. 3. During the latency preceding the Ca2+ spike, the stores increased their sensitivity
to InsP3. This sensitization seemed to be an all-or-none phenomenon. 4. Oxidized glutathione and thimerosal promoted the spontaneous
release by sensitizing the InsP3 receptor. 5. An increase in the [Ca2+] within the stores was required for both the increased
sensitivity to InsP3 and the subsequent spike. 6. Caffeine (6 mM) antagonized the effect of very low InsP3 concentrations
and abolished the Ca2+ spike, without itself releasing Ca2+. 7. Our results suggesting that luminal Ca2+ may sensitive InsP3-sensitive
stores leading to spontaneous Ca2+ mobilization will be discussed in the light of a modified version of the two-pool model
for explaining cytosolic Ca2+ oscillations.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.1992.sp019319</identifier><identifier>PMID: 1484365</identifier><identifier>CODEN: JPHYA7</identifier><language>eng</language><publisher>Oxford: The Physiological Society</publisher><subject>Action Potentials - physiology ; Animals ; Biological and medical sciences ; Caffeine - pharmacology ; Calcium - metabolism ; Cell Membrane Permeability ; Fundamental and applied biological sciences. Psychology ; Liver - cytology ; Liver - metabolism ; Mitochondria, Liver - metabolism ; Rats ; Rats, Wistar ; Reaction Time ; Stomach ; Sulfhydryl Compounds - pharmacology ; Vertebrates: digestive system</subject><ispartof>The Journal of physiology, 1992-09, Vol.455 (1), p.623-640</ispartof><rights>1992 The Physiological Society</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5623-26a7eeb063fc68c84dad7e0e8e471553f71b790e3d0368f734d1029e92ca83b53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1175662/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1175662/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5572958$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1484365$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Missiaen, L</creatorcontrib><creatorcontrib>Taylor, C W</creatorcontrib><creatorcontrib>Berridge, M J</creatorcontrib><title>Luminal Ca2+ promoting spontaneous Ca2+ release from inositol trisphosphate-sensitive stores in rat hepatocytes</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>1. Spontaneous Ca2+ release from the inositol 1,4,5-trisphosphate (InsP3)-sensitive stores in permeabilized hepatocytes was
monitored using Fluo-3 to measure the free [Ca2+] of the medium bathing the cells. 2. Permeabilized cells rapidly sequestered
Ca2+, reducing the [Ca2+] to 103 +/- 5 nM. Under conditions that depended critically upon cell density and the amount of Ca2+
in the medium, this was followed by a slow increase in [Ca2+] culminating in a substantial Ca2+ spike representing synchronous
discharge from the InsP3-sensitive stores. 3. During the latency preceding the Ca2+ spike, the stores increased their sensitivity
to InsP3. This sensitization seemed to be an all-or-none phenomenon. 4. Oxidized glutathione and thimerosal promoted the spontaneous
release by sensitizing the InsP3 receptor. 5. An increase in the [Ca2+] within the stores was required for both the increased
sensitivity to InsP3 and the subsequent spike. 6. Caffeine (6 mM) antagonized the effect of very low InsP3 concentrations
and abolished the Ca2+ spike, without itself releasing Ca2+. 7. Our results suggesting that luminal Ca2+ may sensitive InsP3-sensitive
stores leading to spontaneous Ca2+ mobilization will be discussed in the light of a modified version of the two-pool model
for explaining cytosolic Ca2+ oscillations.</description><subject>Action Potentials - physiology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Caffeine - pharmacology</subject><subject>Calcium - metabolism</subject><subject>Cell Membrane Permeability</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Liver - cytology</subject><subject>Liver - metabolism</subject><subject>Mitochondria, Liver - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reaction Time</subject><subject>Stomach</subject><subject>Sulfhydryl Compounds - pharmacology</subject><subject>Vertebrates: digestive system</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNqNkU-P0zAQxS0EWkrhI4ByQICEUjxxHMcXJKj4q0pwWM6W60war5I42O6u8u1xle4CF8TBsuT3mzfjeYQ8A7oBAPbmaurmYF2_ASmLTZgoSAbyHllBWclcCMnukxWlRZEzweEheRTCFaXAqJQX5ALKumQVXxG3Ow521H221cXrbPJucNGOhyxMbox6RHcMi-SxRx0waxOS2dEFG12fRW_D1Ll0dMQ84Jie7TVmITqPIXGZ1zHrcNLRmTlieEwetLoP-OR8r8mPjx8ut5_z3bdPX7bvdrnhVcHyotICcU8r1pqqNnXZ6EYgxRpLAZyzVsBeSIqsoayqW8HKBmghURZG12zP2Zq8XXyn437AxuAYve7V5O2g_ayctupvZbSdOrhrBSB4lUZYkxdnA-9-HjFENdhgsO-XpajUkhc1FQl89U8QBJVQ14KXCa0W1HgXgsf2bh6g6pSquk1VnVJVt6mmwqd__uZ32RJj0p-fdR2M7luvR2PDHca5KCSvE_Z-wW5sj_N_NleXX7-fHkrOIe0lmbxcTDp76G6sR7WUBWcsxlklToE6kb8AhqLTRQ</recordid><startdate>19920901</startdate><enddate>19920901</enddate><creator>Missiaen, L</creator><creator>Taylor, C W</creator><creator>Berridge, M J</creator><general>The Physiological Society</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19920901</creationdate><title>Luminal Ca2+ promoting spontaneous Ca2+ release from inositol trisphosphate-sensitive stores in rat hepatocytes</title><author>Missiaen, L ; Taylor, C W ; Berridge, M J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5623-26a7eeb063fc68c84dad7e0e8e471553f71b790e3d0368f734d1029e92ca83b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Action Potentials - physiology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Caffeine - pharmacology</topic><topic>Calcium - metabolism</topic><topic>Cell Membrane Permeability</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Liver - cytology</topic><topic>Liver - metabolism</topic><topic>Mitochondria, Liver - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reaction Time</topic><topic>Stomach</topic><topic>Sulfhydryl Compounds - pharmacology</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Missiaen, L</creatorcontrib><creatorcontrib>Taylor, C W</creatorcontrib><creatorcontrib>Berridge, M J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Missiaen, L</au><au>Taylor, C W</au><au>Berridge, M J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Luminal Ca2+ promoting spontaneous Ca2+ release from inositol trisphosphate-sensitive stores in rat hepatocytes</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>1992-09-01</date><risdate>1992</risdate><volume>455</volume><issue>1</issue><spage>623</spage><epage>640</epage><pages>623-640</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><coden>JPHYA7</coden><abstract>1. Spontaneous Ca2+ release from the inositol 1,4,5-trisphosphate (InsP3)-sensitive stores in permeabilized hepatocytes was
monitored using Fluo-3 to measure the free [Ca2+] of the medium bathing the cells. 2. Permeabilized cells rapidly sequestered
Ca2+, reducing the [Ca2+] to 103 +/- 5 nM. Under conditions that depended critically upon cell density and the amount of Ca2+
in the medium, this was followed by a slow increase in [Ca2+] culminating in a substantial Ca2+ spike representing synchronous
discharge from the InsP3-sensitive stores. 3. During the latency preceding the Ca2+ spike, the stores increased their sensitivity
to InsP3. This sensitization seemed to be an all-or-none phenomenon. 4. Oxidized glutathione and thimerosal promoted the spontaneous
release by sensitizing the InsP3 receptor. 5. An increase in the [Ca2+] within the stores was required for both the increased
sensitivity to InsP3 and the subsequent spike. 6. Caffeine (6 mM) antagonized the effect of very low InsP3 concentrations
and abolished the Ca2+ spike, without itself releasing Ca2+. 7. Our results suggesting that luminal Ca2+ may sensitive InsP3-sensitive
stores leading to spontaneous Ca2+ mobilization will be discussed in the light of a modified version of the two-pool model
for explaining cytosolic Ca2+ oscillations.</abstract><cop>Oxford</cop><pub>The Physiological Society</pub><pmid>1484365</pmid><doi>10.1113/jphysiol.1992.sp019319</doi><tpages>18</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Action Potentials - physiology Animals Biological and medical sciences Caffeine - pharmacology Calcium - metabolism Cell Membrane Permeability Fundamental and applied biological sciences. Psychology Liver - cytology Liver - metabolism Mitochondria, Liver - metabolism Rats Rats, Wistar Reaction Time Stomach Sulfhydryl Compounds - pharmacology Vertebrates: digestive system |
| title | Luminal Ca2+ promoting spontaneous Ca2+ release from inositol trisphosphate-sensitive stores in rat hepatocytes |
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