Genomic analysis of heat-shock factor targets in Drosophila

We have used a chromatin immunoprecipitation-microarray (ChIP-array) approach to investigate the in vivo targets of heat-shock factor (Hsf) in Drosophila embryos. We show that this method identifies Hsf target sites with high fidelity and resolution. Using cDNA arrays in a genomic search for Hsf tar...

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Bibliographic Details
Published in:Genome biology 2005-01, Vol.6 (7), p.R63-1098, Article R63
Main Authors: Birch-Machin, Ian, Gao, Shan, Huen, David, McGirr, Richard, White, Robert A H, Russell, Steven
Format: Article
Language:English
Subjects:
Animals
Base Sequence
chromatin
complementary DNA
DNA Primers
Drosophila
Drosophila melanogaster - embryology
Drosophila melanogaster - genetics
Drosophila Proteins - genetics
Embryo, Nonmammalian - physiology
genes
Genomics
heat shock proteins
Heat-Shock Proteins - genetics
Method
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction - methods
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins - genetics
transcription factors
Transcription, Genetic
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Summary:We have used a chromatin immunoprecipitation-microarray (ChIP-array) approach to investigate the in vivo targets of heat-shock factor (Hsf) in Drosophila embryos. We show that this method identifies Hsf target sites with high fidelity and resolution. Using cDNA arrays in a genomic search for Hsf targets, we identified 141 genes with highly significant ChIP enrichment. This study firmly establishes the potential of ChIP-array for whole-genome transcription factor target mapping in vivo using intact whole organisms.
ISSN:1474-760X
1465-6906
1474-760X
1465-6914
DOI:10.1186/gb-2005-6-7-r63