Dicer-1 and R3D1-L catalyze microRNA maturation in Drosophila

In Drosophila melanogaster, Dicer-2/R2D2 and Dicer-1 generate small interfering RNA (siRNA) and microRNA (miRNA), respectively. Here we identify a novel dsRNA-binding protein, which we named R3D1-L, that forms a stable complex with Dicer-1 in vitro and in vivo. While depletion of R3D1-L by RNAi caus...

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Bibliographic Details
Published in:Genes & development 2005-07, Vol.19 (14), p.1674-1679
Main Authors: Jiang, Feng, Ye, Xuecheng, Liu, Xiang, Fincher, Lauren, McKearin, Dennis, Liu, Qinghua
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Amino Acid Sequence
Animals
Base Sequence
Drosophila melanogaster
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Female
In Vitro Techniques
Infertility - genetics
Infertility - metabolism
Male
MicroRNAs - genetics
MicroRNAs - metabolism
Molecular Sequence Data
Mutagenesis, Insertional
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Research Communications
Ribonuclease III - metabolism
RNA Helicases - genetics
RNA Helicases - metabolism
RNA Interference
RNA Processing, Post-Transcriptional
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
Sequence Homology, Amino Acid
Substrate Specificity
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Summary:In Drosophila melanogaster, Dicer-2/R2D2 and Dicer-1 generate small interfering RNA (siRNA) and microRNA (miRNA), respectively. Here we identify a novel dsRNA-binding protein, which we named R3D1-L, that forms a stable complex with Dicer-1 in vitro and in vivo. While depletion of R3D1-L by RNAi causes accumulation of precursor miRNA (pre-miRNA) in S2 cells, recombinant R3D1-L enhances miRNA production by Dicer-1 in vitro. Furthermore, R3D1 deficiency causes miRNA-generating defect and severe sterility in male and female flies. Therefore, R3D1-L functions in concert with Dicer-1 in miRNA biogenesis and is required for reproductive development in Drosophila.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.1334005