The purification and properties of extracellular glycosidases of the cellular slime mould Dictyostelium discoideum

The purification of beta-N-acetylhexosaminidase, alpha-glucosidase, alpha-mannosidase and beta-glucosidase from the spent growth medium of Dictyostelium discoideum strain Ax-2 myxamoebae is described. beta-N-Acetylhexosaminidase and alpha-glucosidase were obtained in high yield and as homogeneous pr...

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Published in:Biochemical journal 1973-05, Vol.133 (1), p.37-47
Main Authors: Every, D, Ashworth, J M
Format: Article
Language:English
Subjects:
Acetamides
Chromatography, DEAE-Cellulose
Chromatography, Gel
Concanavalin A
Drug Stability
Electrophoresis, Polyacrylamide Gel
Electrophoresis, Starch Gel
Enzymology
Glucosidases - isolation & purification
Glucosidases - metabolism
Glycoside Hydrolases - isolation & purification
Glycoside Hydrolases - metabolism
Hexosaminidases - isolation & purification
Hexosaminidases - metabolism
Hydrogen-Ion Concentration
Immunodiffusion
Isoenzymes
Kinetics
Mannose
Molecular Weight
Myxomycetes - enzymology
Nitrophenols
Spectrophotometry
Temperature
Time Factors
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Ashworth, J M
description The purification of beta-N-acetylhexosaminidase, alpha-glucosidase, alpha-mannosidase and beta-glucosidase from the spent growth medium of Dictyostelium discoideum strain Ax-2 myxamoebae is described. beta-N-Acetylhexosaminidase and alpha-glucosidase were obtained in high yield and as homogeneous preparations whereas the alpha-mannosidase preparation consisted of two electrophoretically distinct isoenzymes. The physical, chemical and kinetic properties of these enzymes are described.
doi_str_mv 10.1042/bj1330037
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ispartof Biochemical journal, 1973-05, Vol.133 (1), p.37-47
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1470-8728
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1177668
source PubMed Central
subjects Acetamides
Chromatography, DEAE-Cellulose
Chromatography, Gel
Concanavalin A
Drug Stability
Electrophoresis, Polyacrylamide Gel
Electrophoresis, Starch Gel
Enzymology
Glucosidases - isolation & purification
Glucosidases - metabolism
Glycoside Hydrolases - isolation & purification
Glycoside Hydrolases - metabolism
Hexosaminidases - isolation & purification
Hexosaminidases - metabolism
Hydrogen-Ion Concentration
Immunodiffusion
Isoenzymes
Kinetics
Mannose
Molecular Weight
Myxomycetes - enzymology
Nitrophenols
Spectrophotometry
Temperature
Time Factors
title The purification and properties of extracellular glycosidases of the cellular slime mould Dictyostelium discoideum
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