Problems associated with the assay of arylsulphatases A and B of rat tissues

A method for the separation and purification of rat liver arylsulphatases A and B by gel filtration on Sephadex G-200 is described. The properties of the A enzyme and its molecular weight are similar to those of the corresponding ox liver enzyme. The B enzymes were found to be dissimilar. The method...

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Bibliographic Details
Published in:Biochemical journal 1973-05, Vol.134 (1), p.183-190
Main Authors: Worwood, M, Dodgson, K S, Hook, G E, Rose, F A
Format: Article
Language:English
Subjects:
Animals
Cellular Interactions and Control Processes
Chromatography, Gel
Chromatography, Ion Exchange
Hydrogen-Ion Concentration
Kinetics
Liver - enzymology
Methods
Molecular Weight
Nitrophenols
Rats
Sulfatases - analysis
Sulfatases - isolation & purification
Temperature
Time Factors
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Summary:A method for the separation and purification of rat liver arylsulphatases A and B by gel filtration on Sephadex G-200 is described. The properties of the A enzyme and its molecular weight are similar to those of the corresponding ox liver enzyme. The B enzymes were found to be dissimilar. The method already developed for the assay of the corresponding enzymes from human tissues was shown to be unsuitable for the assay of the enzymes of rat tissues. A method of assay was developed which permits an approximate determination of the individual rat liver enzymes in a mixture of the two, but precise determination requires prior separation of the enzymes by gel-filtration chromatography.
ISSN:0264-6021
0306-3283
1470-8728
DOI:10.1042/bj1340183