Determination and significance of l-tyrosine O-sulphate and its deaminated metabolites in normal human and mouse urine

Methods were developed for the determination of the O-sulphate esters of l-tyrosine, p-hydroxyphenylpyruvate, p-hydroxyphenylacetate and p-hydroxybenzaldehyde in human urine. The amounts of these esters normally present in human female urine were determined. The quantities and specific radioactiviti...

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Bibliographic Details
Published in:Biochemical journal 1973-06, Vol.134 (2), p.629-635
Main Authors: Hext, P M, Thomas, S, Rose, F A, Dodgson, K S
Format: Article
Language:English
Subjects:
Metabolism in Whole Organs
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Summary:Methods were developed for the determination of the O-sulphate esters of l-tyrosine, p-hydroxyphenylpyruvate, p-hydroxyphenylacetate and p-hydroxybenzaldehyde in human urine. The amounts of these esters normally present in human female urine were determined. The quantities and specific radioactivities of l-tyrosine O-sulphate and p-hydroxyphenylpyruvate O-sulphate in mouse urine after labelled tyrosine had been fed were determined and were consistent with the hypothesis that the sole source of p-hydroxyphenylpyruvate O-sulphate was l-tyrosine O-sulphate. It is suggested that the turnover of known polypeptides containing l-tyrosine O-sulphate residues can account for only a portion of the quantities of the ester and its metabolite(s) that are present in normal female human urine.
ISSN:0264-6021
0306-3283
1470-8728
DOI:10.1042/bj1340629