Activation of delta-aminolaevulate synthetase in extracts of Rhodopseudomonas spheroides

1. The spontaneous activation of delta-aminolaevulate synthetase in extracts from Rhodopseudomonas spheroides grown semi-anaerobically in the light requires oxygen and does not take place anaerobically in the dark. 2. Activation is completely prevented by azide or cyanide and is partially inhibited...

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Bibliographic Details
Published in:Biochemical journal 1970-04, Vol.117 (3), p.609-613
Main Authors: Marriott, J, Neuberger, A, Tait, G H
Format: Article
Language:English
Subjects:
Amino Acids
Azides - pharmacology
Citrates - pharmacology
Cyanides - pharmacology
Levulinic Acids
Ligases - analysis
Ligases - antagonists & inhibitors
Malonates - pharmacology
NAD - metabolism
Oxygen Consumption
Rhodopseudomonas - enzymology
Rhodopseudomonas - metabolism
Succinates - pharmacology
Ultrasonics
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Summary:1. The spontaneous activation of delta-aminolaevulate synthetase in extracts from Rhodopseudomonas spheroides grown semi-anaerobically in the light requires oxygen and does not take place anaerobically in the dark. 2. Activation is completely prevented by azide or cyanide and is partially inhibited by chlorpromazine. These compounds inhibit markedly the succinoxidase activity of extracts. 3. NADH delays activation, but when it has been oxidized by the extract activation begins at the normal rate and complete activation occurs. By contrast both the rate and the extent of activation are markedly decreased by even small amounts of carboxylic acids. 4. The inhibitory effects of succinate and citrate on activation can be prevented by malonate and fluorocitrate respectively. 5. These results suggest that for activation to occur some endogenous compound has to be oxidized via the electron transport chain. 6. Activation occurs under anaerobic conditions in the light, probably by photo-oxidation.
ISSN:0264-6021
0306-3283
1470-8728
DOI:10.1042/bj1170609