Phosphatydylglycerol promotes bilayer insertion of salmon calcitonin

Neutron diffraction from oriented multibilayers has been used to study the bilayer interaction of the amphipathic peptide salmon calcitonin. Penetration of calcitonin into bilayers composed of dioleoylphosphatidylcholine increases with the addition of 15% (mol) of the anionic phospholipid dioleoylph...

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Bibliographic Details
Published in:Biophysical journal 1997-05, Vol.72 (5), p.2180-2186
Main Author: Bradshaw, J.P.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Calcitonin - metabolism
Fourier Analysis
Lipid Bilayers - metabolism
Molecular Sequence Data
Phosphatidylcholines - metabolism
Phosphatidylglycerols - metabolism
Water
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Summary:Neutron diffraction from oriented multibilayers has been used to study the bilayer interaction of the amphipathic peptide salmon calcitonin. Penetration of calcitonin into bilayers composed of dioleoylphosphatidylcholine increases with the addition of 15% (mol) of the anionic phospholipid dioleoylphosphatidylglycerol. Neutron scattering profiles of water distribution in stacked bilayers show a continuous band of deuterons across each bilayer, consistent with the suggestion that the hormone forms transbilayer alpha-helixes under these conditions. These experiments add to the growing body of data on the role of phosphatidylglycerol in bilayer insertion of protein helices and suggests a possible evolutionary history for calcitonin.
ISSN:0006-3495
1542-0086
DOI:10.1016/S0006-3495(97)78861-1