Immunocytologic detection of Chlamydia psittaci from cervical and vaginal samples of chronically infected ewes

An immunocytologic method was developed for the detection of chronic Chlamydia psittaci infection from the reproductive tract of ewes. Vaginal and cervical samples from 8 infected and 2 non-infected ewes were stained with a C. psittaci-specific monoclonal antibody. Cells containing C. psittaci were...

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Bibliographic Details
Published in:Canadian journal of veterinary research 1998, Vol.62 (1), p.72-74
Main Authors: Papp, J.R, Shewen, P.E, Thorn, C.E, Andersen, A.A
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Antibody Specificity
carrier state
cervix
Cervix Uteri - microbiology
Cervix Uteri - pathology
Chlamydophila psittaci
Chlamydophila psittaci - isolation & purification
estrous cycle
Estrus
ewes
Female
immunocytochemistry
Immunohistochemistry - methods
latent infections
ovulation
Progesterone - blood
Psittacosis - pathology
Psittacosis - veterinary
Sheep
Sheep Diseases
vagina
Vagina - microbiology
Vagina - pathology
Vaginal Smears
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Summary:An immunocytologic method was developed for the detection of chronic Chlamydia psittaci infection from the reproductive tract of ewes. Vaginal and cervical samples from 8 infected and 2 non-infected ewes were stained with a C. psittaci-specific monoclonal antibody. Cells containing C. psittaci were only detected from the 8 infected ewes and the level of detection varied with respect to the estrus cycle. An increased number of infected cells were observed during the periovulation period, thus indicating an optimal window for detection.
ISSN:0830-9000