Isolation and short term culture of canine adrenal microvascular endothelial cells

A rapid method for the isolation of endothelial cells from canine adrenal glands is described. The technique is based on collagenase digestion of adrenal glands following mechanical tissue disruption. The endothelial cells attach and spread onto plastic petri dishes coated with a commercial attachme...

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Bibliographic Details
Published in:Canadian journal of veterinary research 1999, Vol.63 (1), p.1-4
Main Authors: Trochta, O.A, Jacobs, R.M, Jefferson, B.J
Format: Article
Language:English
Subjects:
adhesion
adrenal glands
Adrenal Glands - cytology
Animals
Cell Culture Techniques - methods
Cell Division
Dogs
endothelium
Endothelium, Vascular - cytology
factor VIII
surface interactions
tissue culture
Veterinary Medicine - methods
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Summary:A rapid method for the isolation of endothelial cells from canine adrenal glands is described. The technique is based on collagenase digestion of adrenal glands following mechanical tissue disruption. The endothelial cells attach and spread onto plastic petri dishes coated with a commercial attachment factor approximately one hour after seeding. Duration to first passage was approximately one week. Doubling times were 2-3 days. Cells were positively identified with immunoperoxidase staining for von Willebrand factor antigen.
ISSN:0830-9000