Reinnervation of the lateral gastrocnemius and soleus muscles in the rat by their common nerve

• Published in: The Journal of physiology 1986-03, Vol.372 (1), p.485-500
• Main Authors: Gillespie, M J, Gordon, T, Murphy, P R
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Biomechanical Phenomena; Female; Fundamental and applied biological sciences. Psychology; Hindlimb - innervation; Male; Motor Neurons - physiology; Muscle Contraction; Muscle Denervation; Muscles - innervation; Muscles - physiology; Nerve Regeneration; Rats; Rats, Inbred Strains; Space life sciences; Striated muscle. Tendons; Time Factors; Vertebrates: osteoarticular system, musculoskeletal system
• ISSN: 0022-3751; 1469-7793
• DOI: 10.1113/jphysiol.1986.sp016021

To determine whether there is any specificity of regenerating nerves for their original muscles, the common lateral gastrocnemius soleus nerve (l.g.s.) innervating the fast-twitch lateral gastrocnemius (l.g.) and slow-twitch soleus muscles was sectioned in the hind limb of twenty adult rats. The proximal nerve stump was sutured to the dorsal surface of the l.g. muscle and 4-14 months later, the contractile properties of the reinnervated l.g. and soleus muscles and their single motor units were studied by dissection and stimulation of the ventral root filaments. Contractile properties of normal contralateral muscles were examined for comparison and motor units were isolated in l.g. and soleus muscles for study in a group of untreated animals. Measurement of time and rate parameters of maximal twitch and tetanic contractions showed that the rate of development of force increased significantly in reinnervated soleus muscles and approached the speed of l.g. muscles but rate of relaxation did not change appreciably. In reinnervated l.g. muscles, contraction speed was similar to normal l.g. muscles but relaxation rate declined toward the rates of relaxation in control soleus muscles. After reinnervation by the common l.g.s. nerve, the proportion of slow motor units in l.g. increased from 10 to 31% and decreased in soleus from 80 to 31%. The relative proportions of fast and slow motor units in each muscle were the same as the proportions of fast and slow units in the normal l.g. and soleus muscles combined. It was concluded that fast and slow muscles do not show any preference for their former nerves and that the change in the force profile of the reinnervated muscles is indicative of the relative proportions of fast and slow motor units: fast units dominate the contraction phase and slow units the relaxation phase of twitch and tetanic contractions of the muscle.