Evidence for acetylcholine receptor blockade by intracellular hydrogen ions in cultured chick myoballs

Acetylcholine (ACh)-induced membrane currents were recorded in voltage-clamped myoballs in culture. The ACh sensitivity and the charge transfer per channel (Qc) were doubled in NH4+ at normal extracellular pH (pHo). Immediately after wash-out of NH4+, the ACh sensitivity and Qc undershot below the c...

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Bibliographic Details
Published in:The Journal of physiology 1983-10, Vol.343 (1), p.429-437
Main Authors: Goldberg, G, Lass, Y
Format: Article
Language:English
Subjects:
Acetylcholine - metabolism
Ammonium Chloride - pharmacology
animal physiology
Animals
Biological and medical sciences
Cell membranes. Ionic channels. Membrane pores
Cell structures and functions
Cells, Cultured
Chick Embryo
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
Intracellular Fluid - metabolism
Membrane Potentials
Molecular and cellular biology
Muscles - metabolism
Receptors, Cholinergic - drug effects
Receptors, Cholinergic - metabolism
Receptors, Cholinergic - physiology
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Summary:Acetylcholine (ACh)-induced membrane currents were recorded in voltage-clamped myoballs in culture. The ACh sensitivity and the charge transfer per channel (Qc) were doubled in NH4+ at normal extracellular pH (pHo). Immediately after wash-out of NH4+, the ACh sensitivity and Qc undershot below the control. The recovery period of the ACh sensitivity and Qc was about 20 min. The ACh sensitivity and Qc greatly increased at alkaline pHo and decreased at acid pHo. We suggest that the intracellular H+ ions can block the ACh-induced channel. The pHo effects may be mediated by a similar (but smaller) change in the intracellular pH (pHi). Intracellular alkalinization can improve the channel function.
ISSN:0022-3751
1469-7793
DOI:10.1113/jphysiol.1983.sp014901