The cell walls of Pseudomonas aeruginosa. General composition

1. Cell walls of Pseudomonas aeruginosa were prepared and analysed. 2. Separate preparations were found to be reproducible, e.g. the phosphorus contents of all batches lay in the range 2.0-2.1%. 3. Ninhydrin-positive compounds in hydrolysates accounted for 43-46% of the cell wall and 79-87% of the n...

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Bibliographic Details
Published in:Biochemical journal 1967-11, Vol.105 (2), p.749-754
Main Authors: Clarke, K, Gray, G W, Reaveley, D A
Format: Article
Language:English
Subjects:
Amino Acids - analysis
Ammonia - analysis
Autoanalysis
Bacterial Proteins - analysis
Carbohydrates - analysis
Cell Wall - analysis
Chromatography, Paper
Fatty Acids - analysis
Hexosamines - analysis
Indicators and Reagents
Nitrogen - analysis
Phosphorus - analysis
Pseudomonas aeruginosa - analysis
Spectrophotometry
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Summary:1. Cell walls of Pseudomonas aeruginosa were prepared and analysed. 2. Separate preparations were found to be reproducible, e.g. the phosphorus contents of all batches lay in the range 2.0-2.1%. 3. Ninhydrin-positive compounds in hydrolysates accounted for 43-46% of the cell wall and 79-87% of the nitrogen of the cell wall. Examination of the results for individual ninhydrin-positive compounds showed that 5-15% of the cell wall was murein and about 30% protein. 4. Trypsin treatment of crude cell-wall preparations preferentially liberated arginine, lysine and leucine, but it was not clear whether these arose from cell-wall or cytoplasmic proteins.
ISSN:0264-6021
0306-3283
1470-8728
DOI:10.1042/bj1050749