Precursors of the pyrimidine moiety of thiamine

1. A method was devised for obtaining the pyrimidine moiety of thiamine in a pure form after its excretion into the medium by de-repressed washed-cell suspensions of mutants of Salmonella typhimurium LT2. 2. By using amino acid-requiring mutants, this excretion of pyrimidine moiety was shown to be d...

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Bibliographic Details
Published in:Biochemical journal 1968-01, Vol.106 (1), p.271-277
Main Authors: Newell, P C, Tucker, R G
Format: Article
Language:English
Subjects:
Amino Acids - metabolism
Carbon Isotopes
Depression, Chemical
Enzyme Repression
Ethionine - pharmacology
Genetics, Microbial
Glycine - metabolism
Methionine - metabolism
Methods
Mutation
Pyrimidines - biosynthesis
Pyrimidines - isolation & purification
Radioisotope Dilution Technique
Salmonella typhimurium - enzymology
Salmonella typhimurium - growth & development
Salmonella typhimurium - metabolism
Thiamine - biosynthesis
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Summary:1. A method was devised for obtaining the pyrimidine moiety of thiamine in a pure form after its excretion into the medium by de-repressed washed-cell suspensions of mutants of Salmonella typhimurium LT2. 2. By using amino acid-requiring mutants, this excretion of pyrimidine moiety was shown to be dependent on the presence of both methionine and glycine. 3. In the presence of either [Me-(14)C]methionine or [G-(14)C]methionine, methionine-requiring mutants did not incorporate radioactivity into the pyrimidine moiety. 4. In contrast, both [1-(14)C]glycine and [2-(14)C]glycine were incorporated into the pyrimidine moiety excreted by glycine-requiring mutants, and this occurred with little or no dilution of specific radioactivity. 5. The possible requirement for methionine as a cofactor and the significance of the incorporation of both carbon atoms of glycine are discussed.
ISSN:0264-6021
0306-3283
1470-8728
DOI:10.1042/bj1060271