Store-operated Calcium Entry Inactivates at the Germinal Vesicle Breakdown Stage of Xenopus Meiosis

Store-operated calcium entry (SOCE) is the predominant Ca 2+ influx pathway in non-excitable cells and is activated in response to depletion of intracellular Ca 2+ stores. We have studied SOCE regulation during Xenopus oocyte meiosis. SOCE can be measured readily in stage VI Xenopus oocytes arrested...

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Bibliographic Details
Published in:The Journal of biological chemistry 2000-12, Vol.275 (49), p.38710-38715
Main Authors: Machaca, K, Haun, S
Format: Article
Language:English
Subjects:
Animals
Calcium - metabolism
Cell Cycle - physiology
Egtazic Acid - analogs & derivatives
Egtazic Acid - pharmacology
Female
G2 Phase
Homeostasis
In Vitro Techniques
Meiosis
Membrane Potentials
Mitosis
Oocytes - cytology
Oocytes - drug effects
Oocytes - physiology
Ovum - cytology
Ovum - drug effects
Ovum - physiology
Patch-Clamp Techniques
Xenopus
Xenopus laevis
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Summary:Store-operated calcium entry (SOCE) is the predominant Ca 2+ influx pathway in non-excitable cells and is activated in response to depletion of intracellular Ca 2+ stores. We have studied SOCE regulation during Xenopus oocyte meiosis. SOCE can be measured readily in stage VI Xenopus oocytes arrested at the G 2 –M transition of the cell cycle, either by Ca 2+ imaging or by recording the SOCE current. However, following meiotic maturation, SOCE can no longer be activated by store depletion. We have characterized the time course of SOCE inactivation during oocyte maturation, and show that SOCE inactivates almost completely, in a very short time period, at the germinal vesicle breakdown stage of meiosis. This acute inactivation offers an opportunity to better understand SOCE regulation.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M007887200