GENETIC ANALYSIS OF MUTANTS WITH A REDUCED Ca2+-DEPENDENT K+ CURRENT IN PARAMECIUM TETRAURELIA

Two mutants of Paramecium tetraurelia with greatly reduced Ca2+-dependent K+ currents have been isolated and genetically analyzed. These mutants, designated pantophobiac, give much stronger behavioral responses to all stimuli than do wild-type cells. Under voltage clamp, the Ca2+-dependent K+ curren...

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Bibliographic Details
Published in:Genetics (Austin) 1985-11, Vol.111 (3), p.433-445
Main Authors: Hinrichsen, Robert D, Amberger, Ed, Saimi, Yoshiro, Burgess-Cassler, Anthony, Kung, Ching
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Calcium - pharmacology
Classical genetics, quantitative genetics, hybrids
Electric Conductivity
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Investigations
Ion Channels - physiology
Mutation
Paramecium - genetics
Paramecium - physiology
Potassium - metabolism
Protozoa
Species Specificity
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Summary:Two mutants of Paramecium tetraurelia with greatly reduced Ca2+-dependent K+ currents have been isolated and genetically analyzed. These mutants, designated pantophobiac, give much stronger behavioral responses to all stimuli than do wild-type cells. Under voltage clamp, the Ca2+-dependent K+ current is almost completely eliminated in these mutants, whereas the Ca2+ current is normal. The two mutants, pntA and pntB, are recessive and unlinked to each other. pntA is not allelic to several other ion-channel mutants of P. tetraurelia. The microinjection of a high-speed supernatant fraction of wild-type cytoplasm into either pantophobiac mutant caused a temporary restoration to the wild-type phenotype.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/111.3.433